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Federal Register / Vol. 59, No. 127 / Tuesday, )iiiy 5, 1999 / Notices 
Appendix P-Il-A-l-c. Decontamination 
and Inactivation (BLl-P) 
Appendix P-II-A-l-c-(l)- 
Experimental organisms shall be 
rendered biologically inactive by 
appropriate methods before disposal 
outside of the greenhouse facility. 
Appendix P-lI-A-l-d. Control of 
Undesired Species and Motile 
Macroorganisms (BLl-P) 
Appendix P-Il-A-l-d-(l). A program 
shall be implemented to control 
undesired species (e.g., weed, rodent, or 
arthropod pests and pathogens), by 
methods appropriate to the organisms 
and in accordance with applicable state 
and Federal laws. 
Appendix P-II-A-l-d-(2). Arthropods 
and other motile macroorganisms shall 
be housed in appropriate cages. If 
macroorganisms (e.g., flying arthropods 
or nematodes) are released within the 
greenhouse, precautions shall be taken 
to minimize escape from the greenhouse 
facility. 
Appendix P-ll-A-l-e. Concurrent 
Experiments Conducted in the 
Greenhouse (BLl-P) 
Appendix P-ll-A-l-e-(l). Experiments 
involving other organisms that require a 
containment level lower than BLl-P 
may be conducted in the greenhouse 
concurrently with experiments that 
require BLl-P containment, provided 
that all work is conducted in accordance 
with BLl-P greenhouse practices. 
Appendix P-ll-A-2. Facilities (BLl-P) 
Appendix P-ll-A-2-a. Definitions (BLl- 
P) 
Appendix P-II-A-2-a-(l). The term 
■greenhouse’ refers to a .structure with 
walls, a roof, and a floor designed and 
used principally for growing plants in a 
controlled and protected environment. 
The walls and roof are usually 
constructed of transparent or 
translucent material to allow passage of 
sunlight for plant growth. 
Appendix P-II-A-2-a-(2). The term 
■greenhouse facility' includes the actual 
greenhouse rooms or compartments for 
growing plants, including all 
immediately contiguous hallways and 
head-house areas, and is considered part 
of the confinement area. 
Appendix P-Il-A-2-b. Greenhouse 
Design (BLl-P) 
Appendix P-II-A-2-b-(l). The 
greenhouse floor may be composed of 
gravel or other porous material. At a 
minimum, impervious (e g., concrete) 
walkways are recommended. 
Appendix P-lI-A-2-b-(2). Windows 
and other openings in the walls and roof 
of the greenhouse facility may be open 
for ventilation as needed for proper 
operation and do not require any special 
barrier to contain or exclude pollen, 
microorganisms, or small flying animals 
(e.g., arthropods and birds); however, 
screens are recommended. 
Appendix P-II-B. Biosafety Level 2 — 
Plants (BL2-P) 
Appendix P-II-B-1. Standard Practices 
(BL2-P) 
Appendix P-II-B-1 -a. Greenhouse 
Access (BL2-P) 
Appendix P-II-B-l-a-(l). Access to the 
greenhouse shall be limited or 
restricted, at the discretion of the 
Greenhouse Director, to individuals 
directly involved with the experiments 
when they are in progress. 
Appendix P-II-B-l-a-(2). Personnel 
shall be required to read and follow 
instructions on BL2-P practices and 
procedures. All procedures shall be 
conducted in accordance with accepted 
greenhouse practices that are 
appropriate to the experimental 
organisms. 
Appendix P-lI-B-l-b. Records (BL2-P) 
Appendix P-ll-B-l-b-(l). A record 
shall be kept of experimental plants, 
microorganisms, or small animals that 
are brought into or removed from the 
greenhouse facility. 
Appendix P-Il-B-l-b-(2). A record 
shall be kept of experiments currently in 
progre.ss in the greenhouse facility. 
Appendix P-II-B-1 -b-(3). The 
Principal Investigator shall report any 
greenhouse accident involving the 
inadvertent release or spill of 
microorganisms to the Greenhouse 
Director, Institutional Biosafety 
Committee, NIH/ORDA and other 
appropriate authorities immediately (if 
applicable). Reports to the NIH/ORDA 
shall be sent to the Office of 
Recombinant DNA Activities, National 
Institutes of Health, Building 31, Room 
4B11, Eethesda, Maryland 20892, (301) 
496-9838. Documentation of any such 
accident shall be prepared and 
maintained. 
Appendix P-lI-B-l-c. Decontamination 
and Inactivation (BL2-P) 
Appendix P-II-B-l-c-(l). 
Experimental organisms shall be 
rendered biologically inactive by 
appropriate methods before disposal 
outside of the greenhouse facility. 
Appendix P-II-B-l-c-(2). 
Decontamination of run-off water is not 
necessarily required. If part of the 
greenhouse is composed of gravel or 
similar material, appropriate treatments 
should be made periodically to 
eliminate, or render inactive, any 
organisms potentially entrapped by the 
gravel. 
Appendix P-ll-B-l-d. Control oi 
Undesired Species and Motile 
Macroorganisms (BL2-P) 
Appendix P-lI-B— l-d-(l). A pjogism 
shall be implemented to control 
undesired species (e.g., weed, rodent, or 
arthropod pests and pathogens) by 
methods appropriate to the organisms 
and in accordance with applicable state 
and Federal laws. 
Appendix P-ll-B-l-d-(2). Arthropods 
and other motile macroorganisms shall 
be housed in appropriate cages. If 
macroorganisms (e.g., flying arthropods 
or nematodes) are released within the 
greenhouse, precautions shall be taken 
to minimize escape from the greenhouse 
facility. 
Appendix P-II-B-l-e. Concurrent 
Experiments Conducted in the 
Greenhouse (BL2-P) 
Appendix P-Il-B— l-e-(l). Experiments 
involving other organisms that require a 
containment level lower than BL2-P 
may be conducted in the greenhouse 
concurrently with experiments that 
require BL2-P containment provided 
that all work is conducted in accordance 
with BL2— P greenhouse practices. 
Appendix P-II-B-l-f. Signs (BL2-P) 
Appendix P-ll-B-l-f-(l). A sign shall 
be posted indicating that a restricted 
experiment is in progress. The sign shall 
indicate the following: (i) the name of 
the responsible individual, (ii) The 
plants in use, and (iii) any special 
requirements for using the area. 
Appendix P-II-B— l-f-(2). If organifms 
are used that have a recognized 
potential for causing serious detrimenta) 
impacts bn managed or natural 
ecosystems, their presence shall be 
indicated on a sign posted on the 
greenhouse access doors. 
Appendix P-lI-B-l-f-(3). If there is a 
risk to human health, a sign shall be 
posted incorporating the universal 
biosafety symbol. 
Appendix P-ll-B-l-g. Transfer of 
Materials (BL2-P) 
Appendix P-II-B-1 -g-(l). Materials 
containing experimental 
microorganisms, which are brought into 
or removed from the greenhouse facility 
in a viable or intact state, shall be 
transferred in a closed non-breakable 
container. 
Appendix P-ll-B-l-h. Greenhouse 
Practices Manual (BL2-P) 
Appendix P-II-B-1 -h-(l). A 
greenhouse practices manual shall be 
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