Federal Register / Vol. 59, No. 127 / Tuesday, July 5, 1994 / Notices 
34515 
and animals, and (ii) before exiting the 
laboratory. 
Appendix G-II-A-l-g. All procedures 
are performed carefully to minimize the 
creation of aerosols. 
Appendix G-II-A-l-h. In the interest 
of good personal hygiene, facilities (e.g., 
hand washing sink, shower, changing 
room) and protective clothing (e.g., 
uniforms, laboratory coats) shall be 
provided that are appropriate for the 
risk of exposure to viable organisms 
containing recombinant DNA 
molecules. 
Appendix G-lI-A-2. Special Practices 
(BLl). Appendix G-II-A-2-a. 
Contaminated materials that are to be 
decontaminated at a site away from the 
laboratory are placed in a durable leak- 
proof container which is closed before 
being removed from the laboratory. 
Appendix G-II-A-2-b. An insect and 
rodent control program is in effect. 
Appendix G-II-A-3. Containment 
Equipment (BLl). Appendix G-II-A-3-a. 
Special containment equipment is 
generally not required for manipulations 
of agents assigned to BLl. 
Appendix G-II-A~4. Laboratory 
Facilities (BLl). Appendix G-II-A— 4-a. 
The laboratory is designed so that it can 
be easily cleaned. 
Appendix G-II-A— 4-b. Bench tops are 
impervious to water and resistant to 
acids, alkalis, organic solvents, and 
moderate heat. 
Appendix G-II-A— 4-c. Laboratory 
furniture is sturdy. Spaces between 
benches, cabinets, and equipment are 
accessible for cleaning. 
Appendix G-II-A-4-d. Each laboratory 
contains a sink for hand washing. 
Appendix G-II-A— 4-e. If the laboratory 
has windows that open, they are fitted 
with fly screens. 
Appendix G-II-B. Biosafety Level 2 
(BL2) (see Appendix G-III-N) 
Appendix G-lI B-1. Standard 
Microbiological Practices (BL2). 
Appendix G-II-B-l-a. Access to the 
laboratory is limited or restricted by the 
Principal Investigator when work with 
organisms containing recombinant DNA 
molecules is in progress. 
Appendix G-II-B-l-b. Work surfaces 
are decontaminated at least once a day 
and after any spill of viable material. 
Appendix G-II-B-l-c. All 
contaminated liquid or solid wastes are 
decontaminated before disposal. 
Appendix G-II-B-l-d. Mechanical 
pipetting devices are used; mouth 
pipetting is prohibited. 
Appendix G-Il-B-l-e. Eating, 
drinking, smoking, and applying 
cosmetics are not permitted in the work 
area. Food may be stored in cabinets or 
refrigerators designated and used for 
this purpose only. 
Appendix G-II-B-l-f. Persons wash 
their hands; (i) after hemdling materials 
involving organisms containing 
recombinant DNA molecules and 
animals, and (ii) when exiting the 
laboratory. 
Appendix G-11-B-l-g. All procedures 
are performed carefully to minimize tlie 
creation of aerosols. 
Appendix G-II-B-l-h. Experiments of 
lesser biohazard potential can be 
conducted concurrently in carefully 
demarcated areas of the same laboratory. 
Appendix G-II-B-2. Special Practices 
(BL2). Appendix G-II-B-2-a. 
Contaminated materials that are to be 
decontaminated at a site away from the 
laboratory are placed in a durable leak- 
proof container which is closed before 
being removed from the laboratory. 
Appendix G-II-B-2-b. The Principal 
Investigator limits access to the 
laboratory. The Principal Investigator 
has the final responsibility for assessing 
each circumstance and determining who 
may enter or work in the laboratory. 
Appendix G-II-B-2-c. The Principal 
Investigator establishes policies and 
procedures whereby only persons who 
have been advised of the potential 
hazard and meet any specific entry 
requirements (e.g., immunization) may 
enter the laboratory or animal rooms. 
Appendix G-II-B-2-d. When the 
organisms containing recombinant DNA 
molecules in use in the laboratory 
require special provisions for entry (e.g., 
vaccination), a hazard warning sign 
incorporating the universal biosafety 
symbol is posted on the access door to 
the laboratory work area. The hazard 
warning sign identifies the agent, lists 
the name and telephone number of the 
Principal Investigator or other 
responsible person(s), and indicates the 
special requirement(s) for entering the 
laboratory. 
Appendix G-ll-B-2-e. An insect and 
rodent control program is in effect. 
Appendix G-II-B-2-f. Laboratory 
coats, gowns, smocks, or uniforms are 
worn while in the laboratory. Before 
exiting the laboratory for non-laboratory 
areas (e.g., cafeteria, library, 
administrative offices), this protective 
clothing is removed and left in the 
laboratory or covered with a clean coat 
not used in the laboratory. 
Appendix G-Il-B-2-g. Animals not 
involved in the work being performed 
are not permitted in the laboratory. 
Appendix G-II-B-2-h. Special care is 
taken to avoid skin contamination with 
organisms containing recombinant DNA 
molecules; gloves should be worn when 
handling experimental animals and 
when skin contact with the agent is 
unavoidable. 
Appendix G-Il-B-2-i. All wastes from 
laboratories and animal rooms are 
appropriately decontaminated before 
disposal. 
Appendix G-Il-B-2-j. Hypodermic 
needles and syringes are used only for 
parenteral injection and aspiration of 
fluids from laboratory animals and 
diaphragm bottles. Only needle-locking 
syringes or disposable syringe-needle 
units (i.e., needle is integral to the 
syringe) are used for the injection or 
aspiration of fluids containing 
organisms that contain recombinant 
DNA molecules. Extreme caution 
should be used when handling needles 
and syringes to avoid autoinoculation 
and the generation of aerosols during 
use and disposal. Needles should not be 
bent, shear^, replaced in the needle 
sheath or guard, or removed from the 
syringe following use. The needle and 
syringe should be promptly placed in a 
puncture-resistant container and 
decontaminated, preferably autoclaved, 
before discard or reuse. 
Appendix G-Il-B-2-k. Spills and 
accidents which result in overt 
exposures to organisms containing 
recombinant DNA molecules are 
immediately reported to the 
Institutional Biosafety Committee and 
NIH/ORDA. Reports to NIH/ORDA shall 
be sent to the Office of Recombinant 
DNA Activities, National Institutes of 
Health, Building 31, Room 4B11, 
Bethesda, Maryland 20892, (301) 496— 
9838. Medical evaluation, surveillance, 
and treatment are provided as 
appropriate and written records are 
maintained. 
Appendix G-II-B-2-1. When 
appropriate, considering the agent(s) 
handled, baseline serum samples for 
laboratory and other at-risk personnel 
are collected and stored. Additional 
serum specimens may be collected 
periodically depending on the agents 
handled or the function of the facility. 
Appendix G-II-B-2-m. A biosafety 
manual is prepared or adopted. 
Personnel are advised of special hazards 
and are required to read and follow 
instructions on practices and 
procedures. 
Appendix G-II-B-3. Containment 
Equipment (BL 2). Appendix G-ll-B-3-a. 
Biological safety cabinets (Class 1 or II) 
(see Appendix G-III-L) or other 
appropriate personal protective or 
physical containment devices are used 
whenever: 
Appendix G-II-B-3-a-(l). Procedures 
with a high potential for creating 
aerosols are conducted (see Appendix 
G-III-O). These may include 
centrifuging, grinding, blending, 
vigorous shaking or mixing, sonic 
disruption, opening containers of 
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