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Federal Register / Vol. 59, No. 127 I Tuesday, July 5, 1994 / Notices 
materials whose internal pressures may 
be different from ambient {wessures, 
intraneisal inoculation of animals, and 
harvesting infected tissues from animals 
or eggs. 
Appendix G-II-B-3-a-{2). High 
concentrations or large volumes of 
organisms containing recombinant DNA 
molecules are used. Such materials may 
be centrifuged in the open laboratory if 
sealed beads or centrifuge safety cups 
are used and if they are opened only in 
a biological safety cabinet. 
Appendix G-II-B-4. Laboratory 
Facilities (BL 2). Appendix G-Il-B-4-a. 
The laboratory is designed so that it can 
be easily cleaned. 
Appendix G-U-B— 4-b, Bench tops are 
impervious to water and resistant to 
adds, alkalis, organic solvents, and 
moderate heat. 
Appendix G-ITB— 4-c. Laboratory 
furniture is sturdy and spaces between 
benches, cabinets, and equipment are 
accessible for cleaning. 
Appendix G-II-B— 4^. Each laboratory 
contains a sink for hand washing. 
Appendix G-U-B— 4-e. If the laboratory 
has windows that open, they are fitted 
with fly screens. 
Appendix G-II-B— 4-t An autoclave for 
decontaminating laboratory wastes is 
available. 
Appendix G-Il-C Biosafety Level 3 
(BL3) (see Appendix G-IU-P) 
Appendix G-ff-C-1. Standard 
Micr^iological Practices (BL3). 
Appendix G-II-C-l-a. Work surfaces are 
decontamihated at least once a day and 
after any spill of viable nvaterial. 
Appendix G-II-C-l-b.. All 
contaminated liquid or solid wastes are 
decontaminated before disposal. 
App>endix G-U-C-l-c. Mechanical 
pip>etting devices are used; mouth 
pipetting is prohibited. 
App>endix G-U-C-l-d. Eating, 
drinking, smoking, storing food, and 
applying cosmetics are not p>ermitted in 
the work area. 
Appendix G-D-C-l-e. Persons wash 
their hands: (i) after handling materials 
involving organisms containing 
recombinant DNA molecules, and 
handling animals, and (ii) when exiting 
the laboratory. 
App>endix G-U-C-l-f. All procedures 
are performed carefully to minimize the 
creation of aerosols. 
App)endix G-II-G-l-g. Persons under 
16 years of age shall not enter the 
laboratory. 
App)endix G-II-C-l-h. If exp)eriments 
involving other organisms which 
require lower levels of containment are 
to be conducted in the same laboratory 
concurrently with experiments 
requiring BL3 level physical 
containment, they shall be conducted in 
accordance with all BL3 level laboratory 
practices. 
Appendix G-II-C-2. Special Practices 
(BL3) Appendix G-II-C-2-a. Laboratory 
doors are kept closed when experiments 
are in progress. 
Appendix G-II-C-2-b. Contaminated 
materials that are to be decontaminated 
at a site away from the laboratory are 
placed in a durable leak-proof container 
which is closed before being removed 
from the laboratow. 
Appendix G-II-C-2-c. The Principal 
Investigator controls access to the 
laboratory and restricts access to 
persons whose presence is required for 
program or support purpioses. The 
Principal Investigator has the final 
responsibility fox assessing each 
circumstance and determining who may 
enter or w'ork in the laboratory. 
Appendix G-II-C-2-d. The Principal 
Investigator establishes policies and 
procedures whereby only persons who 
have been advised of the potential 
biohazard, who meet any specific entry 
requirements (e.g., immunization), and 
who comply with all entry and exit 
procedures entering the laboratory or 
animal rooms. 
Appendix G-II-C-2-e. When 
organisms containing recombinant DNA 
molecules or experiniental animals are 
present in the laboratory or containment 
module, a hazard warning sign 
incorporating the universal biosafety 
symbol is posted on all laboratory and 
animal room access doors. The hazard 
warning sign identifies the agent, lists 
the name and telephone number of the 
Principal Investigator or other 
responsible personfs), and indicates any 
special requirements for entering the 
laboratory such as the need for 
immunizations, respirators, or other 
personal protective measures. 
Appendix G-II-C-2-f. All activities 
involving organisms containing 
recombinant DNA molecules are 
conducted in biological safety cabinets 
or other physical containment devices 
within the containment module. No 
work in open vessels is conducted on 
the open bench. 
Appendix G-II-C-2 -g. The work 
surfeces of biological safety cabinets and 
other containment equipment are 
decontaminated when work with 
organisms containing recombinant DNA 
molecules is finished. Plastic-backed 
paper toweling used txi non-perforated 
work surfaces within biological safety 
cabinets facilitates clean-up. 
Appendix G-U-C-2-h. An insect and 
rodent program is in effect. 
Appendix G-U-C-2-i. Laboratory 
clothing that protects street doffing 
(e.g., solid front or wrap-around gowns, 
scrub suits, coveralls) is worn in the 
laboratory. Laboratory clothing is not 
w'om outside the laboratory, and it is 
decontaminated prior to laundering or 
disposal. 
Appendix CIl-C-2-j. Special care is 
taken to avoid skin contamination with 
contaminated materials; gloves should 
be worn when handling infected 
animals and when skin contact with 
infectious materials is unavoidable. 
Appendix G-II-C-2-k. Molded surgical 
masks or respirators are worn in rooms 
containing ejmerimental animals. 
Appendix G-II-C-2-1. Animals and 
plants not related to the work being 
conducted are not permitted in the 
laboratory. 
Appendix G-II-C-2-m. Laboratory 
animals held in a BL3 area shall be 
housed in partial-containment caging 
systems, such as Horsfall units (see 
Appendix G-III-K), open cages placed in 
ventilated enclosures, solid-wall and 
-bottom cages covered by filter bonnets 
or solid-wall and -bottom cages placed 
on holding racks equipped with 
ultraviolet in radiation lamps and 
reflectors. 
Note Conventional caging systems may be 
used provided that all personnel wear 
appropriate personal protective devices. 
These protective devices shall Include at a 
minimum wrap-around gowns, head covers, 
gloves, shoe covers, and respirators. All 
personnel shall shower on exit from areas 
where these devices are required. 
Appendix G-II-C-2-n. All wastes from 
laboratories and animal rooms are 
appropriately decontaminated before 
disposal. 
Appendix G-II-C-2-o. Vacuum lines 
are protected with high efficiency 
particulate air/HEPA filters and liquid 
disinfectant traps. 
Appendix G-II-C-2-p. Hypodermic 
needles and syringes are us^ only for 
parenteral injection and aspiration of 
fluids from laboratory animals and 
diaphragm bottles. Only needle locking 
syringes or disposable syringe-needle 
units (i.e., needle is integral to the 
syringe) are used for the injection or 
aspiration of fluids containing 
organisms that contain recombinant 
DNA molecules- Extreme caution 
should be used when handling needles 
and syringes to avoid autoinoculation 
and the generation of aerosols during 
use and disposaL Needles should not be 
bent, sheared, replaced in the needle 
sheath or guard, or removed from the 
syringe following use. The needle and 
syringe should be promptly placed in a 
puncture-resistant container and 
decontaminated, preferably by 
autoclaving, before discard or reuse. 
Appendix G-II— C— 2-q. Spills and 
accidents which result in overt or 
potential exposures to organisms 
Recombinant DNA Research, Volume 19 
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