Federal Register / Vol. 59, No. 127 / Tuesday, July 5, 1994 / Notices 
34519 
corridors outside the laboratory. The 
water service to the fountain is not 
connected to the back-flow protected 
distribution system supplying water to 
the laboratory areas. 
Appendix G-II-D-4-i. Access doors 
to the laboratory are self-closing and 
locking. 
Appendix G-IT-D— 4-j. Any windows 
are breakage resistant. 
App>endix G-II-D-4-k. A double- 
doored autoclave is provided for 
decontaminating materials passing out 
of the facility. The autoclave door which 
opens to the area external to the facility 
is sealed to the outer wall and 
automatically controlled so that the 
outside door can only be opened after 
the autoclave "sterilization” cycle has 
been completed. 
Appendix G-Il-D-4-1. A pass-through 
dunk tank, fumigation chamber, or an 
equivalent decontamination method is 
provided so that materials and 
equipment that cannot be 
decontaminated in the autoclave can be 
safely removed from the facility. 
Appendix G-Il-D-4-m. Liquid 
effluent from laboratory sinks, biological 
safety cabinets, floors, and autoclave 
chambers are decontaminated by heat 
treatment before being released from the 
maximum containment facility. Liquid 
wastes from shower rooms and toilets 
may be decontaminated with chemical 
disinfectants or by heat in the liquid 
waste decontamination system. The 
procedure used for heat 
decontamination of liquid wastes is 
evaluated mechanically and biologically 
by using a recording thermometer and 
an Indicator microorganism with a 
defined heat susceptibility pattern. If 
liquid wastes from the shower room are 
decontaminated with chemical 
disinfectants, the chemical used is of 
demonstrated efficacy against the target 
or indicator microorganisms. 
Appendix G-II-Et-^n. An individual 
supply and exhaust air ventilation 
system is provided. The system 
maintains pressure differentials and 
directional airflow as required to assure 
flows inward from areas outside of the 
facility toward areas of highest potential 
risk within the facility. Manometers are 
used to sense pressure differentials 
between adjacent areas maintained at 
different pressure levels. If a system 
malfunctions, the manometers sound an 
alarm. The supply and exhaust airflow 
is interlocked to assure inward (or zero) 
airflow at all times. 
Appendix G-II-E>-4-o. The exhaust 
air from the facility is filtered through 
high efficiency particulate air/HEPA 
filters and discharged to the outside so 
that it is dispersed away from occupied 
buildings and air intakes. Within the 
facility, the filters are located as near the 
laboratories as practicable in order to 
reduce the len^ of potentially 
contaminated air ducts. The filter 
chambers are designed to allow in situ 
decontamination before filters are 
removed and to facilitate certification 
testing after they are replaced. Coarse 
filters and HEPA filters are provided to 
treat air supplied to the facility in order 
to increase the lifetime of the exhaust 
HEPA filters and to protect the supply 
air system should air pressures become 
unbalanced in the laboratory. 
Appendix G-II-D— 4-p. The treated 
exhaust air from Class 1 and U biological 
safety cabinets may be discharged into 
the laboratory room environment or the 
outside through the facility air exhaust 
system. If ejdiaust air from Class I or II 
biological safety cabinets is discharged 
into the laboratory the cabinets are 
tested and certified at six-month 
inter\'als. The exhaust air from Class III 
biological safety cabinets is discharged, 
without recirculation through two sets 
of high efficiency particulate air/HEPA 
filters in series, via the facility exhaust 
air system. If the treated exhaust air 
from any of these cabinets is discharged 
to the outside through the facility 
exhaust air system, it is cormected to 
this system in a manner (e.g., thimble 
unit connection (see Appendix G-ni-L)) 
that avoids any interference with the air 
balance of the cabinets or the facility 
exhaust air system. 
Appendix G-II-Ef-4-q. A specially 
designed suit area may be provided in 
the facility. Personnel who enter this 
area shall wear a one-piece positive 
pressure suit that is ventilated by a life- 
support system. The life-support system 
includes alarms and emergency backup 
breathing air tanks. Entry to this mea is 
through an airlock fitted with airtight 
doors. A chemical shower is provided to 
decontaminate the surface of the suit 
before the worker exits the area. The 
exhaust air from the suit area is filtered 
by two sets of high efficiency particulate 
air/HEPA filters installed in series. A 
duplicate filtration unit, exhaust fan, 
and an automatically starting emergency 
power source are provided. The air 
pressure within the suit area is greater 
than that of any adjacent area. 
Emergency lighting and communication 
systems are provided. All penetrations 
into the internal shell of the suit are 
sealed. A double-doored autoclave is 
provided for decontaminating waste 
materials to be removed from the suit 
areas. 
APPENDtx G— Table i.— Possible Alte«native Combinations of Physical and Bkxjoqical Containment Safeguards 
Oassrficafton ol physical & biological conlainmoni 
Alternate physical contairwrient 
Alternate biological con- 
tamment 
Latwalory (acili- 
ties 
Laboratory prac- 
tices 
Laboratory equip- 
ment 
BUVHV2 ...._ 
BL3 
BL3 
BL3 
HV2 
BL3 
BL3 
BL4 
HV1 
BL3/HV1 
BL3 
BL3 
BL3 
HV1 
BL3 
Bt-3 
BL2 
HV2 
BL4/HV1 
BU 
BL4 
B14 
HV1 
BL4 
BL4 
BL3 
HV2 
BL— 6M3*alety Level. 
MV— Mo»i-VecVx S/»iem. 
Appendix G-III. Footnotes and 
References of Appendix G 
Appendix G-III-A. Laboratory Safety 
at the Center for Disease Control, U.S. 
Department of Health, Education, and 
Welfare Publication No. CDC 75-8118, 
Septemljer 1974 
Appendix G-III-B. Biosafety in 
Microbiological and Biomedical 
Laboratories, 3rd edition. May 1993, 
U.S. DHHS, Public Health Service, 
Centers for Disease Control and 
Prevention, Atlanta, Georgia, and NIH, 
Belhesda, Maryland. 
Appendix G-III-C. National Cancer 
Institute Safety Standards for Research 
Involving Oncogenic Viruses, U.S. 
Department of Health, Education, and 
Welfare Publication No. (NIH) 75-790, 
October 1974. 
Appendix G-III-D. National Institutes 
of Health Biohazards Safety Guido, U.S. 
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