Federal Register / Vol. 59, No. 127 / Tuesday, July 5, 1994 / Notices 
34535 
experiments that require BLl-P 
containment, provided that all work is 
conducted in accordance with BLl— P 
greenhouse practices. 
Appendix P-U-A-2. Facilities (BLl— P) 
Appendix P-II-A-2-a. Definitions 
(BLl-P) 
Appendix P-II-A-2-a-(l). The term 
"greenhouse” refers to a structure with 
walls, a roof, and a floor designed and 
used principally for growing plants in a 
controlled and protected environment. 
The walls and roof are usually 
constructed of transparent or 
translucent material to allow passage of 
sunlight for plant growth. 
Appendix P-II-A-2-a-(2). The terra 
"greenhouse facility” includes the 
actual greenhouse rooms or 
compartments for growing plants, 
including all immediately contiguous 
hallways and head-house areas, and is 
considered part of the confinement area. 
Appendix P-II-A-2-b. Greenhouse 
Design (BLl-P) 
Appendix P-Il-A-2-b-(l). The 
greenhouse floor may be composed of 
gravel or other porous materi^ At a 
minimum, Imp>ervious (e.g., concrete) 
walkways are recommended. 
Appendix P-II-A-2-h-{2). Windows 
and other openings in the walls and roof 
of the greenhouse facility may be open 
for ventilation as needed for proper 
operation and do not require any special 
barrier to contain or exclude pollen, 
microorganisms, or small flying animals 
(e.g., arthropods and birds): however, 
screens are reconunended. 
Appendix P-II-B. Biosafety Level 2 — 
Plants (BL2-P) 
Appendix P-Ii-B-1. Standard Practices 
(BL2-P) 
Appendix P-II-B-l-a. Greenhouse 
/uioess (BL2-P) 
Appendix P-II-B-l-a-(l). Access to 
the greenhouse shall be limited or 
restricted, at the discretion of the 
Greenhouse Director, to individuals 
directly Involved with the experiments 
when they are in progress. 
Appendix P-Il-B-l-a-(2). Personnel 
shall be required to read and follow 
instructions on BL2-P practices and 
procedures. All procedures shall be 
conducted in accordance with accepted 
greenhouse practices that are 
appropriate to the experimental 
organisms. 
Appendix P-II-B-l-b. Records (BL2-P) 
Appendix P-II-B-l-b-(l). A record 
shall be kept of experimental plants, 
microorganisms, or small animals that 
are brought into or removed from the 
greenhouse facility. 
Appendix P-II-B-l-b-(2). A record 
shall be kept of experiments currently in 
progress in the greenhouse facility. 
Appendix P-II-B-l-b-(3). The 
Principal Investigator shall report any 
greenhouse accident involving the 
inadvertent release or spill of 
microorganisms to the Greenhouse 
Director, Institutional Biosafety 
Conunittee, NIH/ORDA and other 
appropriate authorities immediately (if 
applicable). Reports to the NIH/ORDA 
shall be sent to the Office of 
Recombinant DNA Activities, National 
Institutes of Health, Building 31, Room 
4B11, Bethesda, Maryland 20892, (301) 
496— 9838.X)ocumentation of any such 
accident shall be prepared and 
maintained. 
Appendix P-IT-B-1— c. Decontamination 
and Inactivation (BL2-P) 
Appendix P-II-B-l-c-(l). 
Experimental organisms shall be 
rendered biologically inactive by 
appropriate methods before disposal 
outside of the greenhouse facility. 
Appendix P-Il-B-l-c-{2). 
Decontamination of run-off water is not 
necessarily required If part of the 
greenhouse is composed of gravel or 
similar material, appropriate treatments 
should be made periodically to 
eliminate, or render inactive, any 
organisms potentially entrapped by the 
gravel. 
Appendix P-II-B— 1—d Control of 
Undesired Species and Motile 
Macroorganisms (BL2-P) 
Appendix P-II-B-l-d-(l). A program 
shall be implemented to control 
undcsired species (e.g.. weed, rodent, or 
arthropod pests and pathogens) by 
methods appropriate to the organisms 
and in accordance with applicable state 
and Federal laws. 
Appendix P-Il-B-l-d-(2). 
Arthropods and other motile 
macroorganisms shall be housed in 
appropriate cages. If macroorganisms 
(e.g., flying arthropods or nematodes) 
are released within the greenhouse, 
prccautioiis shall' be taken to minimize 
escape from the greenhouse facility. 
Appendix P-II-B— 1-e. Concurrent 
Experiments Conducted in the 
Greenhouse (BL2-P); 
Appendix P-II-B-l-e-( 1 ). 
Experiments involving other organisms 
that require a containment level lower 
than BL2-P may be conducted in the 
greenhouse concurrently with 
experiments that require BL2-P 
containment provided that all work is 
conducted in accordance with BL2-P 
greeirhouse practices. 
Appendix P-II-B-l-f. Signs (BL2-P) 
Appendix P-U-B-l-t-(l). A sign 
shall be posted indicating that a 
restricted experiment is in progress. The 
sign shall indicate the following; (i) the 
name of the responsible individual, (ii) 
the plants in use, and (iii) any special 
requirements for using the area. 
Appendix P-II-B-l-f-(2). If 
organisms are used that have a 
recognized potential for causing serious 
detrimental impacts on manag^ or 
natural ecosystems, their presence shall 
be indicated on a sign posted on the 
greenhouse access doors. 
Appendix P-II-B-l.-f-(3). If therfe is a 
risk to human health, a sign shall be 
posted incorporating the universal 
biosafety symbol. 
Appendix P-II-B-l-g. Transfer of 
Materials (BL2-P) 
Appendix P-II-B-l-g-(l). Materials 
containing experiment^ 
microorganisms, which are brought into 
or removed horn the greenhouse facility 
in a viable or intact state, shall be 
transferred in a closed non-breakable 
container. 
Appendix P-U-B-l-h. Greenhouse 
Practices Manual (BL2-P) 
Appendix P-lI-B-l-4i-(l). A 
greenhouse practices manual shall be 
prepared or adopted. This manual shall: 
(i) advise personnel of the potential 
consequences if such practices are not 
followed, and (ii) outline contingency 
plans to be implemented in the event of 
the unintentional release of organisms. 
Appendix P-II-B-2. Facilities (BL2-P) 
Appendix P-II-B-2-a. Definitions 
(BL2-P) 
Appendix P-II-B-2-a-(,l). The term 
“greenhouse" refers to a structure with 
walls, a roof, and a floor designed and 
used principally for growing plants in a 
controlled and protected environment. 
The walls and roof are usually 
constructed of transparent or 
translucent material to allow passage of 
sunlight for plant growth- 
Appendix P-IT-B— 2-a-(2l. The term 
"greenhouse facility"Tncludes the 
actual greenhouse rooms or 
compartments for growing plants, 
Including all immediately contiguous 
hallways and head-house areas and is 
considered part of the confinement area. 
Recombinant DNA Research, Volume 19 
