Vector Sequence 
7.1.1 Overall Structure of the tgAAVCF Vector 
The tgAAVCF vector is diagrammed below in Figure 7.1.1. The vector consists of the left- 
hand AAV2 ITR; a full length human CFTR cDNA; a synthetic polyadenylation sequence 
based on the mouse B-globin polyadenylation sequence; AAV2 sequences downstream of the 
cap coding sequence; and the right-hand AAV2 ITR. The vector is propagated in bacteria using 
the bla and ori regions of pBR322. Derivation of the construct is described in section 7.1.3; 
sequence of the construct is provided in Appendix 7.2. 
Figure 7.1.1 
tgAAVCF vector 
pBR322 
'T O 
Q. 
CFTR 
cDNA 
RNA start at nucleotide 128 of ITR 1 
Translation start at nucleotide 133 of CFTR cDNA 
Translation termination at nucleotide 4573 of CFTR cDNA 
Polyadenylation signal in synthetic poly A site nucleotide 8 
Plasmid backbone is pBR322 based containing Amp^ and ori 
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Recombinant DNA Research, Volume 20 
