FF Principal Investigator/Program Director (Last, first, middle): 
BIOGRAPHICAL SKETCH 
Give the following information for the key personnel and consultants and collaborators. Begin with the principal 
investigator/program director. Photocopy this page for each person. 
CVs 
NAME 
Terence R. Flotte, M.D. 
POSITION TITLE 
Assistant Professor of Pediatrics 
_ JUCATION (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.) 
DEGREE 
YEAR 
CONFERRED 
B . S . , Summa 
1982 
M.D. 
1986 
Resid. 
1989 
Post-doc . 
1992 
Post-doc . 
1992 
INSTITUTION AND LOCATION 
FIELD OF STUDY 
Univ. of New Orleans, New Orleans, LA 
Louisiana State Univ., New Orleans, LA 
Johns Hopkins Univ., Baltimore, MD 
N. I .H. (NIDDK) , Bethesda, MD 
Johns Hopkins Univ., Baltimore, MD 
Biological Sciences 
Medicine 
Pediatrics 
Molecular Virology 
Pediatric Pulmonology 
RESEARCH AND PROFESSIONAL EXPERIENCE: Concluding with present position, list, in chronological order, previous employment, experience, and 
honors. Key personnel include the principal investigator and any other individuals who participate in the scientific development or execution of the project. 
Key personnel typically will include all individuals with doctoral or other professional degrees, but in some projects will include individuals at the masters or 
baccalaureate level provided they contribute in a substantive way to the scientific development or execution of the project. Include present membership on 
any Federal Government public advisory committee. List, in chronological order, the titles, all authors, and complete references to all publications during the 
past three years and to representative earlier publications pertinent to this application. DO NOT EXCEED TWO PAGES. 
07/01/93 to Present: 
07/01/92 to 06/30/93: 
07/01/89 to 6/30/92: 
0//01/86 to 06/30/89: 
--Research Assistant Professor of Pediatrics, Eudowood Division of 
Pediatric Respiratory Sciences , Johns Hopkins University 
Co-director , Vector Core Facility of Johns Hopkins Cystic Fibrosis 
Gene Therapy Center 
— Co- investigator, Johns Hopkins CF Gene Therapy Center 
--Instructor of Pediatrics/Leroy Matthews Physician Scientist (CF 
Foundation) , Eudowood Division of Pediatric Respiratory Sciences, Johns 
Hopkins University 
Co-investigator , Johns Hopkins CF Research Development Program 
--Pediatric Pulmonary Fellow, Johns Hopkins/NIH/Cystic Fibrosis 
Foundation Fellowship, Eudowood Division of Respiratory Sciences, Johns 
Hopkins University 
--Post-doctoral Research Fellow, Laboratory of Molecular and Cellular 
Biology (LMCB) NIDDK, NIH, Bethesda, MD 
--Pediatric Residency Training, The Harriet Lane Pediatric House Staff 
of the Johns Hopkins Hospital 
GRANTS AWARDED 
therapy -*- ns ^tutes °f Health (NHLBI) P01-HL51811 "Adeno-associated virus vectors for CF gene 
October 1993 to present 
--Cystic Fibrosis Foundation Leroy Matthews Physician Scientist Award 
July 1991 to June 1995 
;:^:i^T Stlg ^° r . ° n Fibrosis Research Development Project of Johns Hopkins Medical 
Institutions (Cystic Fibrosis Foundation) 
February 1992 to present 
—Cystic Fibrosis Foundation/NIH Fellowship Award 
July 1989 to June 1991 
1. PUBLICATIONS 
i: , . plobto Afione , SA ' Solow R, McGrath SA, Conrad C, Zeitlin PL, Guggino WB, Carter BJ. 
stable In Y1VQ expression of the cystic fibrosis transmembrane conductance regulator with an 
90?10613-10617 Sd VirUS VeCtor * Proceed i nqs of ills national Academy af Sciences USA 1993; 
2. Flotte TR, Afione S, Zeitlin PL, Solow R, Markakis D, Guggino WB, Carter BJ. Expression of 
n2L C 7 StlC T fibro . sls , transmembrane conductance regulator from a novel adeno-associated virus 
promoter. J ourna l o£ Biological chemistry 1993; 268:3781-3790. 
E 5fH M ' plotto . TR '. Afione S, Solow R, Zeitlin PL, Carter BJ, Guggino WB. Correction of 
aetective PKA regulation of outwardly rectifying chloride channels by insertion of cystic 
3 58^58 1-5 g ^ ransineinbrane conductance regulator into CF airway epithelial cells. Nature 1992; 
TR. Prospects for virus-based gene therapy for cystic fibrosis (invited review, 54 
refs. ) . Journal Of Bioenorgetics and Biomembranes 1993; 25:33-38. 
TR and Zeitlin PL. Adeno-associated virus vector gene expression in bronchial 
■ecJi^ Rfnin^ \ S 994 (in e pt e ess) 0f CeU division ' ^ E o sp i ratorv Coll and 
Recombinant DNA Research, Volume 20 
[157] 
