Appendix A2 . 1 Construct of Ad^CFTR.10 (cont.) 
Map Units 
0 10 ZO 30 40 50 60 70 80 90 100 
i — T 
t — i — i — r 
1.25 9.2 
Ad 5 
Ad GV CFTR.10 
SV40 CFTR CMV 
polyA cDNA promoter 
and ipitdng 
signal* 
T 
T 
T 
78.5 B4.7 
Appendix A2.1.2. Schematic of Ad^CFTR-lO. Recombinant adenoviral vector 
AdcvCFTR.10 containing the human CFTR cDNA. Shown schematically are the 
wildtype adenovirus type 5 (Ad5) genome and the details of the CFTR expression 
cassette of Ad^CFTR-lO. Ad5 DNA is divided into 100 map units (mu; 100 mu = 
36 Kb) . The recombinant vector was constructed from the Ad5 derived virus 
Addl324, which is missing the majority of the Ela and Elb and a portion of the 
E3 regions. The Ad^CFTR.10 expression cassette includes (3' to 5 ' ) : the left 
454 bp of the Ad5 genome [including the sequences of the inverted inverted 
terminal repeat (ITR) , the origin of DNA replication, the encapsidation signal 
and the enhancer of the Ela promoter] , followed by the CMV enhancer /promoter , 
splice sequences, CFTR cDNA, and the SV40 early polyadenylation signal. 
[ 222 ] 
Recombinant DNA Research, Volume 20 
