Lymphocyte Gene Therapy for Mild Hunter Syndrome 
Fig. 3 - Correction Of Glycosaminoglycan Metabolism In LCLmps-L2SN 
The ability of this high-level expression in LCL to mediate correction of lysosomal 
metabolism in co-cultivated, untransduced cells was also tested. Diploid fibroblast cultures 
established from patients with Hunter syndrome were studied by labeling of glycosaminoglycan 
with 35S0 4 . Co-cultivated L2SN-transduced LCLmps were more effective at preventing 35s04 
accumulation into Hunter fibroblasts than control virus (LXSN) transduced LCLmps or normal 
LCL (Fig. 4). 
[ 288 ] 
Recombinant DNA Research, Volume 20 
