treatment will improve your heart and breathing functions. 
(5) Determine if there are any other effects of this new form of 
treatment (i.e., watch for other improvements and for side- 
effects of the treatment). 
Procedures : 
If you agree to be in this study, we ask that you do the following 
things : 
(1) Apheresis To remove some of your lymphocyte blood cells, we 
ask that you have two needles inserted into veins in your arm. 
Blood will be pumped through one of these needles through a 
small tube into a machine. This machine will remove some of 
your lymphocytes from the blood. The blood will then leave the 
machine through another small tube and be pumped back into your 
blood stream through the other needle. To remove enough 
lymphocytes, you would be connected to the machine for about 3 
hours. This process does not cause any pain except for the 
brief sting that occurs when the needles are first put into 
your arm. Lymphocytes will be removed once every month for 1 
year, in other words, a total of 12 times. 
(2) Reinfusion Each time some of your lymphocytes are removed, 
they will be grown in the laboratory for 8 days and exposed to 
the virus. These treated lymphocytes will then be returned to 
your blood stream by injection through an "IV" into a vein in 
your arm. This infusion will take about 2 hours. This process 
does not cause any pain except for the brief sting that occurs 
when the needle is first put into your arm. Lymphocytes will 
be injected once every month for 1 year, in other words, 12 
times . 
(3) Blood samples After injection of your genetically modified 
lymphocytes, 5 blood samples will be taken. Each blood sample 
will be 1 Tablespoon. These samples will be used to measure the 
amount of IDS enzyme, and to count the genetically modified 
lymphocytes in your body. 
(4) Urine samples Every month, we will ask you to collect a 24- 
hour urine sample to measure the amount of storage material . 
(5) Other tests Every 3 months, we will ask you to have a regular 
physical examination, and to give a blood sample (of 2 
Tablespoons) for a series of tests. We will also ask you to 
have some other painless, routine medical tests to learn how 
your body is responding to gene therapy treatment. These tests 
are: (1) a "pulmonary function test" during which you blow into 
a machine to evaluate your breathing strength and volume; (2) 
a "CT scan" of your abdomen during which you lie very still for 
approximately 2 0 minutes so that the machine can calculate the 
size of your liver and spleen; (3) an "EKG" during which wires 
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