Figure 4 
PCR Amplification of Thymidine 
Kinase in Cells and Tumors 
i T 
Markers (872 bp and 603 bp) 
H 2 O Negative Control 
% 
Genomic REN DNA 
1 
Ad.RSVtk Plasmid 
5 1 
Un-transduced REN Cells 
1 
MOI: 0.001“ 
* t . 
MOI: 0.01 
: i i . 
MOI: 0.1 
Ad.RSVtk-transduced 
1 i 
MOI: 1.0 
REN Cells 
i i 
MOI: 10 
I 
MOI: 100 _ 
1 1 * 
Ad.RSVtk transduced Tumor 
Un-transduced Tumor 
1 
Markers (872 bp and 603 bp) 
1 

Figure 5 
RT-PCR of Organs and Tumors of Animals Infected with Ad.RSVT/C 
1. HSVTK-Infected REN tumor Cells* (pos. control) 
2. TK plasmid (pos. control) 
3. No Template added (negative control) 
4. Uninfected Tumor Cells* 
5. Brain 
6. Skeletal Muscle 
7. Heart 
8. Liver 
9. Salivary Gland 
1 0. Heart (second specimen) 
1 1 . Lung 
12. Tumor Nodule 
13. Kidney 
14. DNA Markers 
1 
l 
1 
1 
1 
X 
1 
i : 
1 
K 
I 
• 
, in ' • 
» 
; 
HS VTK Primers p-actin Primers 
* No p-actin primers run in this lane 
Recombinant DNA Research, Volume 20 
[403] 
