Appendix D 
Vector Sequence 
i 
Recombinant adenoriruses 
^Plaque purification 
Ad5CMV-p53 tiral stocks 
Figure 1. Scheme for generation of recombinant p53 adenovirus. The p53 
expression cassette was inserted between the Xba I and Cla I sites of 
pXCJL.1. The p53 expression vector (pEC53) and the recombinant 
plasmid pJM17 were cotransfected into 293 cells. The transfected cells 
were maintained in medium until the onset of the cytopathic effect. 
Identification of newly generated p53 recombinant adenoviruses 
(Ad5CMV-p53) by PCR analysis of the DNA samples prepared from the 
cell culture supernatants was described elsewhere24 
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