Appendix D 
/BamHI 
• " i. r 
' * d&Y 
H 0.86 kb H 
mtm ^ii.- ■ > ■ -z. 
' ' 1 
mmmm 
tejSfe 
liiiiis 
y -.{gu 
Figure 2. Structural analysis of Ad5CMV-p53 DNA. (A) A map of Ad5CMV-p53 genomic 
DNA, showing the locations of the p53 expression cassette, the PCR primers, 
and the restriction sites. (B) An agarose gel analysis of PCR products. Two 
pairs of primers that define 1.4-kb (p53) and 0.86-kb (Ad5) DNA fragments 
were used in each reaction. DNA templates used in each reaction were 
pEC53 plasmid (lane 1), Ad5/RSV/GL2 DNA (lane 2), no DNA (lane 3), and 
Ad5CMV-p53 DNA (lane 4). (C) Restriction mapping of Ad5CMV-p53 DNA. 
CsCI-gradient purified Ad5CMV-p53 DNA samples were digested with no 
enzyme (U), Hind III (H), Bam HI (B), Eco Rl (E), and Cla I (C), respectively, 
and analyzed on 1% agarose gel. 
Recombinant DNA Research, Volume 20 
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