Fig. 2 The transduction efficiency of adenovirus in C 6 glioma cells in vitro was demonstrated 
by using a recombinant adenoviral vector containing the bacterial bgal gene. The bgal 
enzyme contains a nuclear translocation signal and results in dense nuclear staining. C 6 
cells (5 x 10 5 ) were plated on 1.5 cm diameter wells, transduced with ADV-bgal at various 
viral doses, and stained with X-gal 48 hours later. A, M.O.I. = 0; B, M.O.I. = 125 C MOI 
= 500 and D, M.O.I. = 2000. 
> v -v* • * - 
ADV Transduction of Human Neural Cell Lines 
Fig. 3 ADV-bgal transduction of glial cells of human origin. The cell lines are: A172 
glioblastoma (■), A4 neuroglioma (•); SW1088 astrocytoma (▼); U373 glioblastoma (▲). 
Recombinant DNA Research, Volume 20 
