ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
133 
to twenty-two genera ; tlie genus which is far the best represented is 
Miliolina of Williamson. The author appears to have collected at ten 
stations. 
Trypanosomata of Mammalia.* — Dr. J. Rouget has studied Trypano- 
somata for two years and a half from a series of inoculations in different 
animals, the original source being a Barb stallion which died of the 
disease called “ dourine.” Trypanosoma , as observed in the blood of the 
horse, is an extremely mobile wormlike body composed of homogeneous 
protoplasm, bounded by an undulating membrane, and having at its 
anterior extremity a small bright sphere. It is from 18-26 p long and 
from 2-2*5 p broad. Morphologically it resembles the parasites de- 
scribed by Lewis, Evans, and Chalachnikow as occurring in the blood of 
mules, horses, camels, and rats. 
The author’s attempts at artificial cultivation were unsuccessful. 
With direct transference from animal to animal there was marked 
success in the case of white and grey mice, white rats, rabbits, and 
dogs, while others, such as snakes, lizards, frogs, birds, and bats, were 
refractory. Mice offer the best field for the study of the parasite, which 
swarms in the blood a few hours after inoculation. In rabbits, dogs, and 
horses there is marked affection of the genitalia. The parasite was 
never found in sections of viscera and organs of animals affected with 
the disease. Very good preparations of the parasite are easily made in 
blood-films on cover-glasses, the best stain being eosin and methylen- 
blue after Chenzinski’s method. The best fixative is alcohol, or equal 
parts of ether and alcohol. 
* Ann. Inst. Pasteur, x. (1896) pp. 716-28 (3 figs.). 
