ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
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solutions. Introduced into the stomach, the remedy has no effect ; in 
order to obtain a reliable effect it must bo injected subcutaneously. For 
this purpose we have used exclusively the small syringe suggested by me 
for bacteriological work ; it is furnished with a small india-rubber ball, 
and has no piston. This syringe can easily be kept aseptic by absolute 
alcohol, and to this we attribute the fact that not a single abscess has 
been observed in the course of more than a thousand subcutaneous 
injections. The place chosen for the injection — after several trials of 
other places — was the skin of the back between the shoulder-blades and 
the lumbar region, because here the injection led to the least local 
reaction — generally none at all — and was almost painless. 
Effect of Injections in Healthy Individuals. — As regards the effect 
of the remedy on the human patient, it was clear from the beginning of 
the research that in one very important point the human being reacts to 
the remedy differently from the animal generally used in experiments — 
the guinea-pig — a new proof for the experimenter of the all-important 
law that experiment on animals is not conclusive for the human being, 
for the human patient proved extraordinarily more sensitive than the 
guinea-pig as regards the effect of the remedy. A healthy guinea-pig 
will bear 2 cubic centimetres, and even more, of the liquid injected sub- 
cutaneously without being sensibly affected. But in the case of a full- 
grown, healthy man 0 • 25 cubic centimetre suffices to produce an intense 
effect. Calculated by body weight, the 1500th part of the quantity, 
which has no appreciable effect on the guinea-pig, acts powerfully on 
the human being. The symptoms arising from an injection of 0*25 
cubic centimetre I have observed after an injection made in my own 
upper arm. They were briefly as follows : — Three to four hours after 
the injection there came on pain in the limbs, fatigue, inclination to 
cough, difficulty in breathing, which speedily increased. In the fifth 
hour an unusually violent attack of ague followed, which lasted almost 
an hour. At the same time there was sickness, vomiting, and rise of 
bodily temperature up to 39 • 6° C. After twelve hours all these symptoms 
abated. The temperature fell until next day it was normal, and a feeling 
of fatigue and pain in the limbs continued for a few days, and for exactly 
the same period of time the site of injection remained slightly painful 
and red. The lowest limit of the effect of the remedy for a healthy 
human being is about 0*01 cubic centimetre (equal to 1 cubic centimetre 
of the hundredth solution), as has been proved by numerous experiments. 
When this dose was used, reaction in most people showed itself only by 
slight pains in the limbs and transient fatigue. A few showed a slight 
rise of temperature up about to 38° G. Although the dosage of the 
remedy shows a great difference between animals and human beings — 
calculated by body weight — in some other qualities there is much 
similarity between them. The most important of these qualities is the 
specific action of the remedy on tuberculous processes, of whatever 
kind. 
The Specific Action on Tuberculous Processes. — I will not here describe 
this action as regards animals used for experiment, but I will at once 
turn to its extraordinary action on tuberculous human beings. The 
healthy human being reacts either not at all, or scarcely at all — as we 
have seen — when O’Ol cubic centimetre is used. The same holds good 
