804 
SUMMARY OF CURBED RESEARCHES RELATING TO 
eight hours they are transferred to hydrochloric and spirit (absolute 
alcohol 100 grm., HC1 10 drops). The acidulated spirit is constantly 
renewed, until the dye is no longer given off, when the sections are 
allowed to remain five to ten minutes longer, after which they are 
removed to absolute alcohol for twenty-four hours. Then bergamot oil 
and xylol dammar. 
By the foregoing method the author claims that elastic fibres are 
well stained, but only if the solution be made in the manner prescribed. 
Demonstrating the finer structural relation of the Liver * * * § — Herr 
A. Oppel applies Golgi’s method to the liver as follows : — A small piece of 
rabbit’s liver is treated with bichromate of potash quickly increased from 
two to five per cent. In three weeks’ time the piece is placed in 3/4 per 
cent, nitrate of silver solution. In a few days the ultimate bile-ducts 
are stained. 
The author gives the result of his procedure with monochromate of 
potash (0*5 per cent.) and silver nitrate on objects kept in spirit for a 
long time. Three per cent, solution of bichromate and 0*5 per cent, 
chromic acid were, however, found to show the biliary network quite as 
well. 
Killing and hardening Pelagic Animals.t — Herr B. Friedlaender 
finds, from the experience of a few months, that the most efficacious fluid 
for killing sea animals (Siplionophora, &c.) is a mixture of water 1000, 
zinc sulphate 125, copper sulphate 125. The solution is placed in one 
vessel, and the animals in sea water in another of similar size. The 
contents of the former are simply poured into the latter vessel. 
For hardening, a 1 per cent, solution of osmic acid in sea water is 
recommended, while for delicate objects osmic acid may be added or 
even used alone as a 1/5 per cent, solution. 
Preserving lower Organisms in Microscopical Preparations.! — 
Pure blood-serum is recommended by Dr. W. Migula as a suitable medium 
for examining and preserving delicate animal and vegetable objects. He 
uses the commercial blood-serum, and filters in an ice-box. through 
bibulous paper frequently changed. The filtrate is mixed with 10 per 
cent, pure glycerin and incubated at 45° to 50° G. When all the water 
has been evaporated, the glycerized jelly is preserved in stoppered 
vessels. When required for use, a small quantity is dissolved in 10 to 
15 times its volume of distilled water, and a large drop placed on the 
slide. Into this drop the living organism is pipetted and then the slide 
is placed in an incubator at about 50°, in order to thicken down the 
fluid. When of the right consistence, the cover-glass, moistened with a 
mixture consisting of 40 parts glycerin, 20 parts absolute alcohol, and 
40 parts water, is imposed. The preparation is again heated for a couple 
of hours, and then ringed round. 
Preparing Blood of Arthropoda and Mollusca.§— According to Sig. 
G. Cattaneo the methods usually adopted for examining the blood of 
* Anat. Auzeig., v. (1890) pp. 143-5. Cf. Zeitschr. f. Wiss. Mikr., vii. (1890) 
pp. 222-3. t Biol. Centralbl., x. (1890) pp. 483-91. 
X Zeitschr. f. Wiss. Mikr., vii. (1890) pp. 172-4. 
§ Bollet. Scient. di Pavia, xi. (18S9) pp. 3-29, 33-57 (2 pis.). Cf. Zeitschr. f. 
Wies. Mikr., vii. (1890) pp. 213-5. 
