ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
819 
hydrochloric or oxalic acid alcohol. They are then placed in water for 
fifteen minutes. The sections are next momentarily immersed in 
ammonia-carmine solution, then in water, and lastly placed for ten to 
fifteen minutes in absolute alcohol, to which 2 per cent, of formic acid 
has been added. After this they are transferred to picric acid alcohol 
for fifteen minutes. Thus prepared the sections have a bluish-green 
hue; the gregarinae are greenish, the cells blue, and the rest of the 
tissue rose-coloured. 
Carmine Stains for Normal and Pathological Preparations.* — Dr. 
K. Haug prepares a double carmine stain for staining pieces in toto as 
follows : — 2 grm. of carmine are rubbed up with 4 grm. of borax-carmine, 
and then boiled in a flask with 300 ccm. distilled water until the fluid is 
evaporated down to 280 ccm. After this, and when the solution has 
cooled down a little, from 10 to 15 ccm. of a 10 per cent, solution of acetic 
acid (glacial) are added by means of a pipette. The addition of the acid 
renders the solution transparent and of a bright red hue. Next day it 
is filtered aud some crystals of thymol added. For staining en masse a 
piece of 0*5 cm. in width, two to four days is required. After this it is 
differentiated with hydrochloric acid alcohol (changed every half hour). 
This takes one to four hours. After this it is placed in a mixture of 
picric acid and alcohol for about twelve hours. 
Ammonia-lithia-carmine. — This solution is made by dissolving 3 grm. 
carmine in 100 ccm. of cold saturated carbonate of lithia solution and then 
adding 5 ccm. of ammonia. It stains quickly and deeply. Wash in water 
and then differentiate in hydrochloric acid alcohol. Sections may be 
after-stained by immersing in picric acid alcohol. 
In many cases when the specimen has been hardened in chromic acid, 
the following modification acts well : — 1 to 1 \ grm. carmine and 2 grm. 
bicarbonate of soda are boiled in 150 ccm. of water, and then 10 to 15 ccm. 
of a 5 per cent, glacial acetic acid added. When cold 5 ccm. lithium 
solution. The subsequent treatment as before. 
(5) Mounting-, including Slides, Preservative Fluids, &c. 
A new Pressureless Mounting-clip-t — Mr. T. Pace writes : “ I 
notice Mr. Bryan’s note in the December number of ‘ Science-Gossip,’ 
Fig. 102. 
suggesting a new form of mounting-clip designed to hold the cover-glass 
without pressure, thus being a great improvement on the spring-clip 
* Zeitschr. f. Wiss. Mikr., vii. (1890) pp. 151-2. 
f Science- Gossip, No. 303 (1890) p. 56. 
Fig. 101. 
