ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
561 
diagnosing the presence of glanders in horses by Strauss’ method, a 
method which consists in injecting some of the suspected glandered tissue, 
or of cultivation from this into the peritoneal sac of male guinea-pigs. One 
of the principal results of this method is an affection of the testicle and 
its coverings, which is observable two to three days after inoculation. 
The skin of scrotum is tense, red, slimy ; suppuration often occurs, and 
in the pus may be found the bacilli of glanders. The animals die in 
from four to fifteen days. Both the tunica vaginalis and the body of 
the testicle are affected. 
The method would seem to be both easy and effectual for diagnosing 
glanders, for if the testicles of an animal become inflamed on the second 
or third day after intraperitoneal inoculation, the presumption would be 
that the disease is glanders. 
The glandered material was derived either from the nasal secretion 
or from a piece of the submaxillary gland ; these were either inoculated 
on some cultivation medium, or were rubbed up with bouillon or water 
and then used directly as an injection. 
Aronson, H. — TJeber die Anwendung der colloidalen Thonerde zur Filtration 
bakterienhaltiger Fliissigkeiten. (On the use of Colloidal Clay for the Filtration 
of Fluids containing Bacteria.) Arch. /. Kinderheilk ., XIV. 1891, pp. 54-8. 
(2) Preparing- Objects. 
Investigation of Structure of Pancreas.* — Prof. C. J. Eberth and 
Dr. K. Muller used the pancreas of freshly killed animals. They pre- 
served the organ in Flemming’s or Rabl’s mixture, or in Hermann’s fluid ; 
in Flemming’s mixture, 1 per cent, solution of platinum chloride took 
the chromic acid. Good results were also obtained by the use of a 1/3 
per cent, solution of platinum chloride and Kleinenberg’s picrosulphuric 
acid. The secondary nuclei were not well shown when fixation was 
effected with corrosive sublimate. The hardened glands were imbedded 
in celloidin or paraffin ; the latter is better for those animals in which 
the cells are smaller than in Amphibia. Care, however, must be taken 
in making the sections. Hsematoxylin with eosin, Platner’s nucleus- 
black, Ogata’s hsematoxylic eosin, nigrosin and saffranin were among the 
staining reagents ; Babes’ safranin-anilin-oil is recommended. 
Examination of Ectoparasitic Trematoda.j — Herr C. Dieckhoff 
finds that little is to be learnt from an examination of living Trematoda. 
The worms were generally killed by heated solution of corrosive subli- 
mate, but in a few cases chrom-osmic-acetic acid or Muller’s fluid was 
used. The objects were hardened in alcohol or Muller’s fluid, and were 
all stained with picrocarmine. The serial sections were 0*01 mm. 
thick. 
Preparation of Epiphytic Fungi .{ — For preserving the various 
parts of epiphytic fungi in their natural position while under examination 
under the Microscope, M. A. Gaillard recommends the following process. 
A drop of collodion is first of all dropped on to the fungus ; but the 
* Zeitschr. f. Wiss. Zool., xxxv. Suppl. (1892) pp. 119 and 20. 
f Arch. f. Naturgesch., lvii. (1891) pp. 247 and 8. 
X Bull. Soc. My col. France, vii. (1891) pp. 233-4. See Bot. Centralbl., 1. (1892) 
p. 75. 
1892. 2 Q 
