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SUMMARY OF CURRENT RESEARCHES RELATING TO 
A test-tube filled in tbe usual manner with gelatin or agar and 
plugged with cotton-wool, is drawn out by means of the blow-pipe flame 
just below the cotton-wool plug, until it becomes longer and narrower 
than the tube of a Liborius’ apparatus. This heating does not affect the 
gelatin. Then a small glass tube is drawn out into a capillary tube, a 
few centimetres of the original being left, so that the capillary portion 
is longer than the test-tube. The latter, after the cotton-wool plug has 
been replaced, is sterilized by heat. The nutrient medium having been 
liquefied by immersing the tube in lukewarm water, is inoculated by 
means of the capillary tube. The wide part of the capillary tube is 
then connected with the apparatus for developing gas (H, CO 2 , &c.) and 
its end pushed close down to the bottom of the test-tube. As the gas 
enters the air is driven out, and when this is effectually completed the 
capillary tube is withdrawn and the test-tube sealed up at the narrow 
part. 
