884 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
Species. 
Trigla gurnardus .. 
Gadus morrhua 
„ seglefinus 
,, minutus 
Motella mustella 
Brosmius brosme 
Hippoglossus limandoides 
Bhombus Isevis 
Arnoglossus laterna 
Average Shrinkage, 
mm. 
.. -1447 
.. -1295 
.. -1295 
.. -1143 
.. -990 
.. *1371 
.. -1524 
.. -1371 
.. *1447 
Plcuronectes platessa *914 
Clupea sprattus -1143 
This was certainly the best of the picric solutions. 
(6) Alcohol, 4 parts ; 2 per cent, acetic acid, 4 parts ; spirits of 
camphor, 1 part. The results here were very similar to the preceding 
fluid, but the embryos were not so distinct, owing to the slight opacity 
of the eggs ; on the other hand, the shrinkage was very little. There 
are many objections to a picric solution which are here met. For 
general work, or for preserving large collections of ova, this is 
undoubtedly the best preservative I have used. 
Species. 
Trigla gurnardus 
Gadus morrhua 
„ seglefinus 
„ minutus 
Motella mustella 
Brosmius brosme 
Hippoglossus limandoides 
Bhombus Isevis 
Arnoglossus laterna 
Pleuronectes platessa 
Clupea sprattus 
Average Shrinkage, 
mm. 
•1371 
•1295 
•1295 
•1143 
•914 
•1143 
•1219 
•1143 
•1219 
•914 
•990 
(7) Various mixtures of Kleinenberg’s picro-sulphuric acid were 
tried, but the results in all cases were unsatisfactory. 
(8) Very satisfactory results were obtained with 50 per cent, 
alcohol. The shrinkage was small, the oil-globule, however, was 
indistinct, and the dense opacity is a disadvantage. 
(9) Perenyi’s fluid stained the eggs a very dark violet. Diluted with 
8 parts of 50 per cent, alcohol, very satisfactory results were obtained. 
The shrinkage averaged *1371 mm., aud the embryo in all the species 
experimented with showed well. 
When ova were not permanently required they were allowed to 
remain in a 2 per cent, solution of acetic acid, or 4 parts of the same to 
2 parts alcohol, and 1 part Perenyi’s fluid ; both mixtures gave good 
results. When the embryos were well advanced, they were allowed to 
remain in the former medium uutil considerable distention took place 
— about one hour or less. No effect was noticed upon the embryo until 
four or five hours. 
