ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
887 
Fig. 104. 
main objection to filters is, that after having been used for a few days 
the filtered water contains more germs than the unfiltered. Of course 
all the results were tested bacteriological ly. 
Trambusti’s Culture Appara- 
tus. — We give a figure of this appa- 
ratus (fig. 104), a description of 
which appeared in the last number 
of the Journal (p. 691). 
Keeping the Inoculation Wire.* — 
Dr. H. C. Plaut suggests that medical 
men desirous of obtaining cultivations 
of disease germs can keep the inocu- 
lating platinum wire and glass rod, 
previously sterilized of course, within 
the test-tube containing the culti- 
vation medium, the test-tube being 
plugged with cotton-wool and covered 
with a caoutchouc cap. 
Method for Cultivating Anaerobic 
Bacteria.! — Herr Hesse cultivates 
anaerobic bacteria in test-tubes on 
solid media in the following way : — 
In a test-tube already filled with a 
solid medium, cotton- wool is loosely 
pushed in for a distance of some cm., the open end of the tube is 
then immersed under mercury, and hydrogen introduced into the tube. 
The tube is then withdrawn, the cotton-wool removed, the medium inocu- 
lated, and then the open end again immersed under the mercury, and 
hydrogen again introduced. For liquid media, or those which may 
become so, and for plate cultivations, a bell-jar is used. 
Apparatus for Cultivating Anaerobic Bacteria.! — Dr. A. H. C. 
van Senus uses a very simple and convenient apparatus for cultivating 
anaerobic bacteria. In a glass tube, the diameter of which is about 
6 mm., a U-shaped bend is made, and one end drawn out to a point. 
The narrow end having been covered, and the wide end plugged with 
cotton, the apparatus is sterilized. To fill the tube the narrow end is 
inserted in gelatin or agar, previously inoculated, and the medium 
sucked in through the broad end. When sufficient has reached the 
U-shaped bend the narrow end is sealed up. In order to obtain a 
colony for inoculation purposes the tube is carefully cleaned with 
H 2 S0 4 and sterilized water, and then having been notched with a 
sterilized file, the piece on which the colony desired is situated, is 
removed. The disadvantage of the method is that it does not afford 
any chance for microscopical observations, although a hand lens is 
available. But by a modification the device may be adapted to plate 
cultivations, by simply blowing a bulb in a tube with diameter of about 
* Centralbl. f. Bakteriol. u. Parasitenk., xii. (1892) p. 203. 
f Zeitschr. f. Hygiene, xi. No. 2. See Centralbl. f. Bakteriol. u. Parasitenk., 
xii. (1892) p. 173. 
% Centralbl. f. Bakteriol. u. Parasitenk., xii. (1892) pp. 144-5. 
