CYTOPLASMIC INCLUSIONS OF THE GERM-CELLS. 557 
•only intend to review Ancel and the subsequent writers. 
(Bolls Lee is mentioned in the text.) 
Since I have not examined embryonic stages I give Ancel’ s 
conclusions somewhat fully. 
Ancel (2) describes the genital rudiment as appearing in 
the embryo several days before hatching. At first the mass 
of cells, quite solid, slowly elongates, and ultimately fuses 
with the hermaphrodite canal. Soon afterwards a lumen 
appears in the solid mass of cells, which now appear like an 
irregular cubical epithelium lining an opening, and these cells 
become covered on their outside by a layer of the mesoderm 
cells in which they lie. At different points inside the lumen 
the genital cells begin to divide mitotically, and in the region 
of these buds secondary, tertiary, etc., culs-de-sac make 
their appearance. In this way the hermaphrodite gland is 
built up from a solid rudiment. 
The cells which line this gland consist of a single layer. 
Here and there some of the indifferent cells augment in 
volume, the chromatin lumps of the nucleus fuse with one 
another, and give rise to more or less round structures. Ancel 
calls these cells “ cellules progerminatives indifferentes.” 
Part of the chromatin of these cells loses its affinity for 
nuclear stains, and the chromatin lumps become more 
numerous and become rounded. They are now united two by 
two by nuclein filaments. 
All the chromatin of the indifferent progerminative cell 
condenses into several round bodies. The whole cell grows 
in size, the nucleus more than the cytoplasm. This cell is 
called by Ancel the male progerminative cell. The latter 
begins to divide indirectly, the chromosome number being 
forty-eight. The products of these divisions drop into the 
lumen of the ovotestis ; they are large and pedicellate (pedi- 
cule), and have the nucleus in the larger part of the cell. 
These primary spermatogonia go on dividing and give rise to 
much smaller bodies — the secondary spermatogonia. The 
products of this activity soon fill the lumen of the ovotestis, 
and in the germinal epithelium changes take place. The 
