CYTOPLASMIC INCLUSIONS OF THE GERM-CELLS. 573 
They lie in, or are grouped so as to enclose, an archoplasmic 
region near the nucleus. 
Despite Bolls Lee's assertion, I must confess that I am quite 
unable to find a centrosome inside this archoplasm, but there is 
little doubt that such a body may be embedded in this mass. 1 
When growth stage has finished the cell may be the size 
drawn in PI. 33, fig. 33, at 4250 diameters. The first matu- 
ration prophases are in progress. The chromosomes are 
appearing, while the Nebenkern, as such, has disappeared. 
In favourable examples it is found that small rodlets are still 
visible here and there ( N.K . in PI. 33, fig. 33), and these are 
almost certainly parts of the scattered Nebenkern. The 
individual rods appear to break up into minor rodlets. 
Bolls Lee ( 9 ) described in the maturation division a re- 
markable centrosome structure. I found this quite easily in 
some of my preparations, and these triradiate bodies are seen 
at A.S. The striae figured by Lee in these bodies I did not 
find so marked, but as our technique was different this is not 
a matter of great importance. 
The mitochondria are very dense and numerous. The 
spindle now forms, with the disappearance of the astral body 
(A.S.), and one gets a figure in which the mitochondria are 
heaped around the spindle and elongated in shape as if 
affected by some lines of force (see PI. 33, fig. 34). 
Murray ( 8 ) figures the Nebenkern fragments as grouped 
around the poles of the astral figure, but I am unable to come 
to a definite opinion on this point. I am able to say that 
occasionally one finds little rectangular bodies which might 
be the Nebenkern, but unfortunately, as the mitochondria 
also become elongate, it is difficult to come to a decision as to 
the nature of these elements. Since mitochondrial stains also 
tinge the Nebenkern, staining tests have so far failed. The 
second maturation division closely resembles the first in so 
far as the behaviour of the plasmatic bodies are concerned. 
The spermatid then appears as drawn in PI. 33, fig. 35, when 
it is just beginning to metamorphose. At what is later seen 
1 Subsequent work shows that Lee is correct. 
