ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
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methyl-green, methyl-blue, gentian-violet, safranin, Bismarck-brown, 
eosiu, fuchsin, tropeolin, and malachite-green may be used in the above 
ways. 
Magalhaes, P. S. de. — E studo geral das colora 9 oes em histologia. (Use of 
staining-methods in histology.) Kio de Janeiro, 1889, 8vo, 89 pp. 
Feist, B. — Ueber die vitale Methylenblaufarbung markbaltiger Nervenstamme. 
(On inethylen-blue staining of living medullated nerve-fibres.) 
Strassburg, 1889, 8vo. 
Kkysinsky, S. — Beitrage zur histologiscben Technik. 5. Knpfercarmin. 6. 
Lithiumcarmin und Lithiumpikrincarmin. (Contributions to histological tech- 
nique. 5. Copper-carmine. 6. Lithium-carmine and lithium-picrocarmine.) 
VircAow’s Arc/i., CXVII. (1889) pp. 204-6. 
Martinotti, G. — Alcuni miglioramenti nella tecnica della reazione al nitrate 
d’ argento nei centri nervosi. (Some improvements in the technique of the 
silver nitrate reaction on the nervous centres.) 
AUi del 12. Congr. della Assoc. Med. dial., I., p. 179. 
]\I 0 NT I, — . — Una nuova reazione degli elementi del sistema nervoso centrale. (A new 
reaction of the elements of the central nervous system.) 
Atti della R. Accad. del Lined — Rendic., V. (1889) p. 705. 
Weigert, C. — Neue Neurogliafarbung. (New neuroglia-staining.) 
Milnchener Med. Wochenschr.., XXXVI. (1889) No. 29. 
(5) Mounting-, including- Slides, Preservative Fluids, «&c. 
Mounting in Glycerin Jelly.* — Glycerin jelly, says Mr. J. D. King, 
answers more purposes as a mounting medium than any other, but it is 
dreaded by many on account of the difficulty of getting rid of air- 
bubbles, a difficulty which may be avoided by the following method. 
Heat the jelly in a water-bath till the water boils, then, always working 
in a warm room, mount with it as you would with glycerin except 
dipping the cover in fluid, being careful to remove any stray air-bubbles 
under the dissecting glass before putting on the cover, for even very 
small ones cannot be depended on to disappear of their own accord. 
Small or delicate objects can be arranged and kept in place by first 
covering the bottom of the cell with glycerin jelly and placing the 
objects in it, being careful to cover them well, and leaving them to 
harden. When hardened, apply additional jelly and put on the cover. 
After standing overnight in a cool place, if the jelly be of good quality 
it may be cleaned off under water with a small paint-brush and finished 
off with cement. It is the better way to use cells for glycerin jelly 
mounts, though it is not necessary to fill the surface or apply the 
cement before putting on the cover-glass, or even in all cases to have 
them as deep as the object is thick. A cell prevents the cover-glass 
from touching the slide at any point, and thus creating a liability of 
forming a vacuum by shrinkage, and it makes better work every way. 
New Mounting Medium.t — Mr. H. Shimerhas devised the following 
mounting medium, the use of which gives every satisfaction. It is a 
mixture of equal parts of glycerin jelly, Farrant’s solution, and glycerin. 
The glycerin jelly is made as follows : — Gelatin, 30 parts ; water, 
70 parts; glycerin, 100 parts; carbolic acid, 2 parts. 
Of this glycerin jelly, liquefied by the heat of a water-bath, pour 
1 fluid oz. into a 4-oz. glass-stoppered bottle, add an equal volume 
of the Farrant’s medium and of glycerin. A little gentle agitating in 
* Mu-ruso(»pc, ix. (1889) p. 138. f T. c., pp. 143-5. 
