ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
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in Miiller’s fluid, and after two or three days they are cut down to slices 
of 1-2 mm. thick. These slices are immersed in Marchi’s fluid (Muller’s 
2 parts, 1 per cent, osmic acid 1 part) for 8 days. After removal they 
are washed, then hardened in alcohol, and imbedded in celloidin. The 
sections are now placed for about 5 minutes in a mixture of Liq. fcrri 
perchlor. 1 part, H 2 0 1 part, rectified spirit 3 parts, and then in Weigert’s 
lisematoxylin solution, which is heated for a few minutes. The fluid 
must not boil, as the celloidin is easily crumpled. After washing in 
water they are differentiated by Pal’s method. The oxalic acid is 
neutralized by washing in ammoniated water, by which the colour is 
often intensified. The nerve -fibres are stained dark brown or black. 
The nuclei of the ganglion cells are a blackish brown. 
Ehrlich’s Triple Stain.* — Dr. G. Reinbach gives a formula for the 
triple stain as improved by Ehrlich. The author used it for staining 
cover-glass preparations of blood. Its composition is as follows: — 
Saturated aqueous solutions of orange G, 120 grm. ; acid rubin, 80 grm. ; 
methyl-green, 100 grm. ; H 2 0, 300 grm. ; absolute alcohol, 180 grm. ; 
glycerin, 50 grm. The aqueous solutions must be saturated. The 
mixture is not to be shaken, but the necessary quantity should be pipetted 
off. The fixed cover-glass film is treated with the solution for 5 to 10 
minutes ; the superfluous stain is washed off with distilled water, the 
surface dried with blotting-paper, and the preparation mounted in balsam. 
Flagella-Staining.f — Herr R. Bunge obtained very good results from 
using cultures which had been incubated for 24 hours and then kept at 
the room temperature for another two days ; even cholera spirilla were 
very successful. The preparations must not be too strongly fixed with 
heat. Instead of carbol-fucbsin, carbol-gentian-violet was used. The 
mordant used was that already noticed in this Journal. t One very im- 
portant result of the author’s experiments was that a differential diagnosis 
between B. typhosus and B. coli commune could not be made by the 
number of flagella, for the latter microbe often had more than the former. 
The author was also able to demonstrate at the same time in the organisms 
examined by him, Proteus , B. coli , B. typh. 3 cholera, subtilis, both flagella 
and capsules. The method was as follows : — The cover-glass prepara- 
tions are first treated as already described. § They are then immersed 
for 1/2-1 minute in 5 per cent, acetic acid, after which they are washed 
and dried. The preparations are thereupon mordanted three or f<;ur 
times, the washing being done after the manner of Nicolle and Morax. 
This over, the preparations are dried, and then stained with gentian- 
violet ; this step is followed by 1/2-1 minute in 1 per cent, acetic acid. 
After this, they are washed in water and dried. A three days’ old culture 
is best suited for this procedure. 
Boeck, C. — Neues Verfahren bei der Farbung der Mikroparasiten auf der Ober- 
flache des Kbrpers. (New Pjocess in Staining Micro-parasites on the Surface of 
the Body.) Mtsh.f. Praltt. Dermatol ., 1894, pp. 467-70. 
* Arch. f. Klin. Chirurg., xlvi. (1893) pp. 486-562 (1 pi.). See Zeitschr. f. whs. 
Mikr., xi. (1894) pp. 258-60. 
f Fortschr. d. Med., xii. (1894) No. 17. See Centralbl. f. Bakteriol. u. Paia- 
sitenk., xvi. (1894) pp. 700-1. J 1894, p. 640. § Loc, cit. 
1895 K 
