ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
327 
New American Species of Sarcosporidia.* * * § — Dr. C. W. Stiles has 
sent to the Zoological Society of France a resume of a paper com- 
municated to the ‘ Veterinary Magazine ’ of Philadelphia. He draws 
attention to the very frequent occurrence of a very delicate species of 
Miescheria in the heart of an American domestic animal ; to a Sarco- 
sporidian from rabbits ; to another intra-muscular Sarcosporidian found 
in the rats of Iowa ; in some localities 75 per cent, were infested by it ; 
to another intra-muscular Sarcosporidian found in chickens ; and to a 
species of Balbiania found in Sethopaga ruticilla. 
Coccidia.f — M. P. Thelolian finds that the plasma of Coccidia may 
exhibit various differentiations. It may contain (1) plastic granules — 
spherical, refractive, and constantly present reserve-products ; (2) large 
refractive globules ; (3) chromatoid granules, of minute size, super- 
ficial position, and strong affinity for hmmatoxylin ; and (4) exceptional 
fatty globules. 
He describes Coccidium cristalloides sp. n. from the intestinal walls 
of Motella ; G. variabile sp. n. from the rectum, &c. of many Teleosteans, 
e. g. Gobius bicolor ; C. clupearum sp. n. from herring, sardine, and 
anchovy ; and a doubtful species from Labrus festivus. 
Protozoan Parasite on Fishes.f — The note of Dr. C. W. Stiles on 
the appearance of Ichthyophthirius multifilis on fishes preserved in 
the aquaria at the Universal Exposition of Chicago has been translated 
into French. This parasite, which has been observed by various authors 
in various places, was submitted by Dr. Stiles to a close investigation. 
He finds that this species was the cause of a considerable mortality 
among the fishes exhibited at Chicago. It appears to have been intro- 
duced by young cat-fish. It is believed to multiply by simple division, 
or by fission into a number of small ciliated tubes. But the two 
methods of reproduction are hardly distinct, as numerous gradations are 
to be seen between them. Reproduction does not only take place at 
night, as was supposed by Herbert, it is also effected during the day. 
Ency station may be produced on the fish or after the parasite has 
quitted it. It is not easy to kill parasites while they are on the fish, 
for fluids tolerated by the fish do not penetrate the viscous material 
which surrounds the parasites. The most practical method for destroy- 
ing the parasite consists in attacking it during its free stage, either 
before or after encystation or during the encysted stage. The last, 
according to the author’s observations, lasts for about one day. The 
young cells which result from the division which goes on during 
encystation swim freely in the water, and then attack new hosts. 
Parasites of Variola.§ — Sig. G. P. Piana and Sig. B. Galli-Valerio 
describe various bodies which they have observed in purulent pocks 
when examined directly, (a) Spherical hyaline immobile corpuscles of 
2-4 /x in diameter ; (6) spherical bodies of 5-7 /x diameter with hyaline 
nucleus and finely granular protoplasm, and one or more refractory 
* Bull. Soc. Zool. France, xix. (1894) p. 160. 
t Arch. f. Zool. Exper., ii. (1894) pp. 541-73 (1 pi.). 
I Bull. Soc. Centr. d’ Agriculture de France, vi. (1894) pp. 165-7 (1 pi.). 
§ La Riforma Med., 1894, No. 126. See Centralbl. f. Bakteriol. u. Parasitenk., 
l e Abt., xvii. (1895) p. 260. 
