ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 377 
Funnel for filling Test-tubes with Media.* — Dr. J. J. van Hesfc 
uses the following apparatus for distributing cultivation media in test- 
tubes, &c. : — Fig. 67 shows an enamelled or glass funnel, attached to the 
pipe of which is a caoutchouc tube b , which can be closed by a pincli- 
cock. To the tube is joined an outflow glass tube c for dipping into the 
test-tube. When the medium is 
poured in through the funnel its flow 
is stopped at any desired point by 
the pinch-cock. Smearing of the 
tube and consequent damage to the 
apparatus for cultivation often hap- 
pens, but this may be avoided by the 
simple device shown in fig. 69, which 
is nothing more than a second tube 
placed between c and the test-tube.. 
Over the end of c is pushed a 
caoutchouc ring (fig. 67) at a dis- 
tance of 4-6 mm. from the end. 
The apparatus shown in fig. 68 can 
be used without any special practice, 
but that in fig. 67 requires some ac- 
quaintance, at least for manipulating 
quickly. 
Modified Papin’s Digester.^ — 
Dr. J. J. Van Hest has used for 
some years a steriliser which can 
reach 100°-130°. The apparatus 
consists of a cylindrical iron kettle 
with an iron top. The steam is 
produced from heating a small 
quantity of water. The lid is fixed 
on by means of a number of small 
screw clamps, and between the pan 
and its cover is a thin layer of 
rubber for the purpose of effectually 
closing the apparatus. In the 
middle of the lid is a valve which 
can be loaded with leaden discs. 
The pieces to be sterilised are placed on a sieve or stand at the 
bottom of the apparatus. The top is then screwed on and the valve 
kept oj)en until the thermometer marks 100°. By this time all the air 
has been driven out, and the valve is then closed by placing on it as 
many lead discs as are necessary to attain the desired temperature. 
When this is reached, the excess of steam escapes through the valve. 
The temperature is pretty constant. 
(2) Preparing- Objects. 
Intercellular Bridges. J — Herr H. Boheman fixed smooth muscle- 
cells with Heidenhain s corrosive sublimate, Kultschitzky’s solution of 
* Centralbl. f. Bakteiiol. u. Parasitenk., l e Abt., xvii. (1895) pp. 462-3 (3 figs.). 
f Tom. cit., pp. 463-4 (1 fig.). $ Anat. Anzeig., x. (1894) pp. 305-15 (6 figs.).’ 
