ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
483 
turn to the right and to the left. Thus a long narrow passage is left in 
the medium. The aspirator is now set going at the rate of 1 litre 
every 2 or 3 minutes, and when a sufficient volume of air has passed 
the opening at t’ is plugged with cork slightly burnt in the flame. 
Fig. 75. Fig. 76. 
When the flasks reach the laboratory, the gelatin is liquefied at 35°— 
37°. Then the flasks are shaken so as to disseminate the germs 
throughout the medium. The gelatin is allowed to set again, and after 
an incubation of 30 days the colonies are counted. 
Preserving Streptococci Cultures.* — Dr. J. Petruschky records the 
fact that he has kept two cultures of Streptococcus for six months, 
without any loss of virulence, and this was done by merely keeping the 
tubes in an ice safe. 
Abel, R., & A. Drae r — D as Huhnerei als Kulturmedium fur Cholera vibrionen. 
(Fowl's Eggs as Culture Media for Cholera- vibrios.) 
Zeitschr.f. Hygiene , XIX. (1895) pp. 61-74. 
Ausset, E. — De l’influence de la temperature dans 1’analyse bacteriologique des 
eaux. (The influence of Temperature in the Bacteriological Examination of 
Water.) Compt. Bend. Soc. Biol., 1895, No. 3, pp. 58-9. 
Ball, M. Y. — A new Culture Medium for the Bacillus of Diphtheria and other 
Bacteria. Med. News, 1894, p. 581. 
Bonhoff — Untersuchungen fiber Giftbildung verschiedener Vibrionen in Hiihner- 
eiern. (Investigations into the formation of Poisons by various Vibrios in Hen’s 
Eggs.) Arch. f. Hygiene , XXII. (1895) p. 351, 
Frothingham, L. — Laboratory Guide for the Bacteriologist. 
Philadelphia, 1895, 8vo, 61 pp. 
Centralbl. f. Bakteriol. u. Parasitenk., l te Abt., xvii. (1895) pp. 551-2, 
