ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
491 
(4) Staining- and Injecting. 
Slight Modification of Golgi’s Method.* — Prof. A. S. Dogiel lias 
endeavoured to get over the well-known difficulty that blood-vessels and 
the ducts of glands are apt to be impregnated as well as the nerves 
in using Golgi’s method. He injects at the outset with a saturated 
solution of Berlin-blue and gelatin, cools the subject in snow for 
20-30 minutes, cuts off suitable portions, and treats with Golgi’s 
bichromate of potash solution or with Ramon y Cajal’s osmio-bichromic 
mixture. The ordinary procedure follows, or a red injection may be 
used, with subsequent treatment in the osmio-bichromic mixture. The 
injection-colours last for some time and obviate confusion. 
Apparatus for Staining Serial Sections.! — Herr R. Borrmann has 
devised an apparatus for the convenient and rapid staining and further 
Fig. 79. 
manipulation of serial sections. It consists (figs. 79 and 80) of a base 
to support a glass rectangular vessel in which the various fluids are 
placed. From the base proceed two metal uprights a , a ', to which are 
fixed by means of screws c c’ the tray d , supported by means of the pieces 
* Anat. Anzeig., x. (1895) pp. 555-7. 
f Zeitschr. f. wiss. Mikr., xi. (1895) pp. 459-61 (2 figs.). 
