ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
493 
L. Demoor followed Golgi’s method, though they took full advantage of 
the modifications suggested or introduced by other workers with the 
same procedure, such as Ramon y Cajal and Kallius. No details of the 
technique are given. They mention that the photographs, which are 
numerous and excellent, were taken on isochromatic plates with the 
oxyhydrogen light. 
Demonstrating the Parasites of Artificial Carcinoma.* — Prof. F. 
Sanfelice placed the pieces removed from the dog and fowl in alcohol, 
and when hard stained them in toto with lithium-carmine. The pieces 
were then treated with acid alcohol and absolute alcohol, imbedded in 
paraffin, and sectioned. The sections were stuck on with albumen and 
freed from paraffin with xylol, washed with absolute alcohol, and then 
placed in Ehrlich’s fluid for 5-10 minutes. On removal they were 
bathed in distilled water, to which a few drops of 0*5 per cent, oxalic 
acid had been added. The excess of acid was next removed by washing 
with distilled water. The sections were dehydrated in absolute alcohol 
and mounted in xylol-balsam. By this method the parasites are stained 
violet and the tissues red. Another method by which good results were 
obtained was, after sticking the sections on slides with albumen, to 
immerse the preparations in a mixture of equal parts of a 1 per cent, 
aqueous solution of safranin and a 1 per cent, aquoso-alcoholic solution 
of malachite-green for 10-20 minutes. The preparations were, after 
washing in distilled water, placed in O' 5 per cent, oxalic acid for 
2-3 minutes, and then in absolute alcohol until they no longer gave off 
any colour, whereupon they were mounted in xylol-balsam. By this 
method the parasites became green and the tissue elements red. 
Hessert, W. — A Simple Stain for Ciliated Bacteria. 
Chicago Med. Recorder , 1894, pp. 240-2. 
(5) Mounting-, including- Slides, Preservative Fluids, &c. 
New Method for Securing Paraffin Sections to the Slide or Cover- 
glass.'f — Miss Agnes M. Claypole finds that “ Among the many steps to 
be taken in making microscopical preparations, that of securing the 
sections to the slide may seem of minor importance, yet the possibility 
of ultimate successful results depends largely on the complete reliance 
to be placed upon the process by which this step is accomplished. 
Especially is this true of serial sectioning when the disarrangement of 
the sections renders the slide almost worthless. In Lee’s compilation 
of microscopical methods, £ The Microtomist’s Vade-Mecum,’ there are 
about a dozen different processes given for fixing paraffin sections to the 
slide. These processes fall into two natural divisions, those fitted for 
material stained in toto , and those fitted for sections to be stained on the 
slide. Of those belonging to the second group, only a few admit of 
the use of both watery and alcoholic stains, and in most of them heat is 
an essential part of the process. 
Many of the methods involve a previous coating of the slide with a 
substance that has to dry and be again moistened before the sections can 
be arranged upon it, such as collodion, shellac, or a gum preparation. 
* Centralbl. f. Bakteriol. u. Parasitenk., l te Abt., xvii. (1895) p. 631. 
t Proc. Amer. Micr. Soc., xvi. (1894) po. 65-7. 
1895 2 K 
