692 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
£. Technique.* 
Notes on Experimental Technique, f — Dr. E. Centanni uses an 
apparatus for injecting animals (fig. 112), which consists of an ordinary 
rubber bulb A. Into the opening is fitted a metal tube B, having a 
diameter of about 1 cm. It projects about 2 cm., and its end is cut off 
obliquely. From the side of the tube, just where it leaves the bulb, 
another metal tube C comes off at right angles. Its diameter is rather 
less than the former’s, and it is connected with a rubber tube about 50 cm. 
long. D is a hook to attach the apparatus to the operator’s coat. When a 
squirting action is required the opening B is first closed 'with the thumb 
and then the rubber ball squeezed. If aspiration be needed the pro- 
cedure is reversed. If it be necessary to keep up a permanent pressure 
or aspiration a Mohr’s pinchcock is placed on the rubber tube and only 
opened when the bulb is working. The diameter of the bulb may be 
Fig. 112. 
Fig. 113. 
from 3-6 cm., and if thick fluids are being worked with, e. g. glycerin 
extracts, or emulsions of tissues, the wall of the bulb must be very 
thick. 
For collecting serum the author uses an almost spherical bottle 
(fig. 113) with short neck and flat bottom. From the side, at about 2/3 
of the height of the bottle, comes off a short tube B. From the middle 
of the bottom rises up the glass rod A to 2/3 of the height of the bottle. 
After the apparatus has been sterilised, the free end of the tube in the 
jugular vein of an animal is passed through the neck of the bottle and 
blood allowed to flow in until it reaches the level of the side tube B. 
When the serum has properly separated it is removed through the side 
tube, the central glass rod preventing the clot from becoming detached. 
For obtaining thin emulsions or for separating the solid and liquid 
portions of emulsions, the author has devised two instruments which 
filter by aspiration and can be worked with a metal net and with filter 
* This subdivision contains (1) Collecting Objects, including Culture Pro- 
cesses ; (2) Preparing Objects ; (3) Cutting, including Imbedding and Microtomes ; 
(4) Staining and Injecting ; (5) Mounting, including slides, preservative fluids, &c. ; 
(6) Miscellaneous. 
t Centralbl. f. Bakteriol. u. Parasitenk., l te Abt., xviii. (1895) pp. 276-82 
(6 figs.). 
