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the first finger so that the end of the plunger projects from the tip 
of the capillary. The end of the plunger is brought against the 
organism and the pressure of the first finger released, when the 
resiliency of the rubber withdraws the plunger and the organism 
is drawn into the end of the capillary. (If other organisms, or 
debris, lie close against the desired organism, they may be knocked 
away by shooting the plunger in and out by the pressure of the 
forefinger.) The organism is then transferred in the pipette to a 
hollow-ground slide containing a drop of the culture medium, and 
is ejected by a pressure on the plunger-head. It is then examined 
under high power to see that it is absolutely free from foreign 
organisms, picked up with the pipette as before, and transferred to 
the culture-vessel. 
The chief advantages of this instrument are : — 
1. The plunger does away with the drawing in of undesirable 
material by capillarity. 
2. The plunger may be employed to clear other organisms away 
from the organism to be isolated. 
3. The instrument is quick and certain in operation. 
4. It is easily portable. 
: _ 5. It is simple and requires no special attachments. 
6. It is inexpensive, and several can be kept on hand ready for 
instant use in case of breakages. 
