ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
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encystations, and there are certainly double encystations without con- 
jugation. The parasitized worms may have Anoplophrya pacliydrili in 
the intestine and another species in the coelom. J. A. T. 
Spores of Leptotheca ohlmacheri. — R. Kudo {Trans. Amer. Micr. 
Soc ., 1921, 40, 161-7, 1 fig.). Ordinary fixation causes about 14 to 
22 p.c decrease respectively in the sutural diameter and breadth of fresh, 
mature spores of this Myxosporidian. The possibility of calculating 
the dimensions of fresh spores from those of fixed and stained spores is 
discussed. The decrease in the dimensions of spores is due to the 
shrinkage of the entire spore body. Fixation makes the coiled polar 
filament invisible. J. A. T. 
Myxosporidian of Frogs. — R. Kudo {Proc. Amer. Soc. Zool. in 
Anat. Record , 1922, 23, 121). In many specimens of Rana clamitans 
and R. pipiens there was infection with Leptotheca ohlmacheri in the 
kidney. A new host seems to be infected by the mouth. The spores 
germinate in the stomach ; the amoebae migrate into the coelom and 
thence into the kidney, where complicated changes occur. J. A. T. 
New Dermocystidium in Frog. — E. Gtjyenot and A. Naville 
( Revue Suisse Zool., 1922, 29, 133-45, 5 figs.). Members of this 
Four spores of Dermocystidium range connected by delicate filaments. 
genus have been previously observed on the skin of newts and the gills 
of Salmonidse. A new form, D. ranee sp. n., is described from Rana 
temporaria , occurring in cysts in the skin, each cyst bent like a U. The 
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