ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 4 UW 
from one vessel to another and deposited in thoir proper compartment 
without the trouble of marking. 
Fig. 59. 
C4) Staining 1 and Injecting. 
Staining of Micro-organisms which will not colour by Gram’s 
Method.* — M. Nieolle states that the following method produces very 
good results for staining micro-organisms which pick up methylen-blue, 
and especially those of glanders, typhoid, swine fever, pseudo-tuber- 
culosis, fowl cholera, soft chancre : — Stain in Loeffler’s or Kuhne’s blue 
for one to three minutes ; wash in water. Immerse in tannin solution 
1-10 (the effect is almost instantaneous); wash in water. Dehydrate 
in absolute alcohol, oil of cloves or bergamot, xylol, balsam. The micro- 
organisms are better differentiated if after staining the preparations 
are treated with weak acetic acid. 
Rapid Staining of Nervous Tissue by the Weigert-Pal and Iron 
Chloride Methods. Dr. Kaiser says that nervous tissue, brain or cord, 
can be stained very expeditiously according to Weigert’s method in the 
following way : — It is most desirable that the pieces should be hardened 
in a chromic acid solution ; four to six weeks in Muller’s fluid are quite 
sufficient, but it is not necessary that the preparations should be placed 
straight away from the hardening fluid into the alcohol; it is more 
advantageous to merely wash them out and harden in spirit afterwards, 
as in this way preparations are more sensitive for other stains. 
The sections are taken from 70 per cent, spirit in which they have 
been kept, and washed in Weigert’s haematoxylin solution (haematoxylin, 
1 ; alcohol, 10 ; water, 90 ; saturated solution of lithium carbonate, 1). 
They are next placed in a fresh quantity of the same fluid in a watch- 
glass, and then gradually heated until bubbles begin to rise. The 
sections thus stained are next differentiated by Pal’s method : — washing 
in water, immersion for about half a minute in 0 • 25 per cent, solution 
of permanganate of potash, and then in the following solution — oxalic 
acid, 1 ; sodium sulphate, 1 ; water, 200. In the last they remain 
* Ann. Inst. Pasteur, 1892, p. 783. See Centralbl. f. Bakteriol. u. Parasitenk., 
xiii. (1893) p. 501. t Zeitschr. f. whs. Mikr., ix. (1893) pp. 468-70. 
