ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 411 
considerably over-stained, and then decolorized to the point desired by 
acetic acid. After completely washing away the acetic acid, they were 
mounted in glycerin. 
Method for rapid Staining Microbes.* * * § — Dr. J. N. Davalos recom- 
mends as a universal staining fluid for all micro-organisms the following 
modification of Ziehl’s solution: — Fuchsin 0*25, alcohol 10*0, crys- 
tallized carbol 5-0, water 100*0. The solution is to be filtered. Cover- 
glasses are floated on for 1-2 minutes, washed and mounted in balsam. 
Chromatin of Sympathetic Ganglia.f — Dr. F. Yas followed Ni ssl’s 
method. The portions of ganglion removed from an animal recently 
dead were placed in absolute alcohol and imbedded in celloidin. The 
sections were stained in aqueous solution of magenta-red, washed out in 
absolute alcohol, cleared in clove oil, and mounted in Canada balsam. 
Obregia’s Method for Class Purposes.^ — Dr. C. L. Gulland says that 
he finds Obregia’s method, described in the ‘ Neurologisches Centralblatt ’ 
for 1890, is very useful for class purposes. The pieces were imbedded 
in paraffin and cut with a rocker. The bottom of the boat was intended 
for the section surface and the pieces were oriented accordingly. The 
ribbons of sections were transferred to plates coated with the following 
solution: — Sugar candy solution of consistence of syrup, 30 ccm. ; 
absolute alcohol, 20 ccm. ; solution of dextrin of syrupy consistence, 
10 ccm. The plates thus coated should be dried in the air, but pro- 
tected from dust. The plates used were 12 in. by 6 in., in fact as large 
as would go in the oven. They are then stoved at a temperature a 
little above melting point of paraffin. In a few minutes the paraffin 
melts and the sections adhere to the sticky surface. The melted 
paraffin is then dissolved by running plenty of naphtha over, and this 
is followed by strong methylated spirit. The sections are then covered 
with celloidin or photoxylin solution (photoxylin 6 grm., absolute 
alcohol 100 ccm., ether 100 ccm.), but a thin celloidin solution poured 
over the surface, and the excess run off, answers well. The plate is 
dried horizontally, so that the layer is perfectly flat, even, and regular. 
The drying must be slow, otherwise the celloidin shrinks. 
At this point the plates may remain till wanted, as the sugar retains 
sufficient moisture to prevent the section from getting too dry. When 
wanted the plates are simply placed in water whereby the sugar is 
dissolved, and then the ribbons or separate sections can be stained and 
mounted. The author mentions the Ehrlich hsematoxylin and aqueous 
eosin, and these stains were followed by methylated spirit sufficiently 
strong to dehydrate. When dehydrated the sections are placed in creosote 
and cleared up. The sections are at this stage handed round the class, 
and the student then removes the creosote with Weigert’s xylol mix- 
ture (xylol three parts, phenol one part) and mounts in balsam. 
Improved Form of Injection Apparatus.§ — Dr. J. Middlemass 
describes an apparatus for injecting which is easily made and manipu- 
* Crbnica Medico-quirurgica de la Habana, 1892, No. 22. See Centralbl. f. 
Bakteriol. u. Parasitenk., xiii. (1893) p. 291. 
t Arch. f. Mikr. Anat., xl. (1892) pp. 375-89 (1 pi.). 
X Journal of Pathology and Bacteriology, 1. (1893) pp. 391-9. 
§ Tom. cit., pp. 389-90 (4 figs.). 
