Progressive Phases of Spirillum. By Dr. B. L. Maddox. 717 
1 d 2 . Sometimes several of these lines or spaces could be seen either 
faintly or strong]y indicated, or only as a minute notch formed in one 
side of the outer membrane. Passing beyond these delicate primary 
indications the changes become more definite, deep notches formed at 
the ends and sides ; these might be only on one side of the convolution 
as in the right-hand Spirillum No. 2 e 4 , or on opposing sides of con- 
tinuous convolutions, as seen in many of the other figures in both 
Nos. 1 and 2, the interior plasm becoming more or less cut across, and 
corresponding to what may be considered a primary minute segmen- 
, tation or fission stage. Following the changes further, the notch at 
the extremity, generally one extremity only, would be continued right 
across the end, as in No. 1 e, and No. 2 e, and e 1 , thus converting the 
tip into a slightly irregular, as seen in No. 1 g, or a more or less 
round or oval little body, No. 2 e 1 , the flagellum often either adhering 
to it or to the corner of the parent body, or else the notch became 
deeply semicircular and in the hollow was lodged a small bright 
circular body, as seen in No. 2 e 3 . This I am tempted to regard as 
spores forming or formed by segmentation. Occasionally when such 
was slightly removed from that position, a very delicate membrane 
could be seen enclosing the vacated space. 
Sometimes the small nipped-off body would be exactly at the tip, 
as shown in figs. No. 1 e and No. 2 e, the one remaining attached, 
the other separated, but both possessing a flagellum. Now and then, 
when incompletely segmented, the entire object would form very 
unusual figures, as in those marked No. 1 d 1 , where both spirilla 
lying close together have undergone the same change at their tips at 
the same time. The long filaments often showed changes of rather a 
different character. In the middle of the body slight differentiation 
could be observed, and running along the centre for the greater portion 
of the filament minute granulation could be noted, which towards the 
central part became surrounded by a lighter space with a dark out- 
line, the outer edge of the organism being slightly swollen or curved 
opposite these nuclear, query, spore formations. This is shown in 
the figure of the drawing No. 1/, though in the photomicrograph of 
the same object this is very indistinct, as it was found impossible, 
from reasons previously stated, to obtain a sharp photograph. In 
the photomicrograph No. 3 of the colony or group, which it was not 
thought necessary to pencil, it will be seen that many of the outlying 
commas have already taken on the nipping process, and some of the 
tips have actually separated into small round bodies, thus showing 
that the colony, whatever may be the object of its formation, over- 
taken by the conditions possibly brought about by the presence of 
flagellate infusoria, and varieties of bacteria, were passing through the 
same changes as seen in the single organisms. What would have 
resulted to the minuter segments a3 marked off by the numerous 
notches indicated in many of the various figures I am unable to state, 
for on the following day I could only find more numerous motile 
3 d 2 
