SUMMARY OF CURRENT RESEARCHES RELATING TO 
28G 
the same time to harden the collodion. From this point we found it 
somewhat difficult to follow accurately the author’s diffuse directions, 
Fig. 29. 
but it seems that the continuation of the procedure is as follows : — 
Immersion in pure benzin, then in 95 per cent, spirit, then in thick 
collodion. After this they are stained, and thereupon cleared up, first 
in 1 0— 80 per cent, spirit, and finally in carbolxylol. The sections are 
in the end mounted definitely in balsam, or provisionally in paraffin. 
C4) Staining- and Injecting-. 
Metallic Impregnation of the Cornea.*— Prof. F. Tartuferi says 
that the fixed cells of the cornea, even to their most delicate prolonga- 
tions, may be deeply stained by immersing the cornea of some adult 
animal (ox) in a solution of hyposulphite of soda (15 grm. to 100 of 
distilled water) for three days or longer, and keeping it at a temperature 
of about 26°. The preparation is then placed in a vessel containing 
finely powdered chloride of silver and a little pure water, for two days 
or longer. 
If the adult cornea be treated in this manner for a still longer 
period, or if the cornea of a young animal be used, these fixed elements 
are but imperfectly visible, but other details are brought out, for 
example, numerous elastic fibrillse ; while by further variations of the 
foregoing method the isolated elastic fibrillae of the cornea may be 
obtained. The preparations are quite permanent. 
Staining Medullated Nerve -fibres with Hgematoxylin and 
Carmine-t— Prof. N. Kultschitzky now gives more complete details of 
his method for staining sections of the central nervous system.J The 
material is hardened in Erlitzki’s fluid for one or two months, and is 
then placed in running water for one or two days. It is next hardened 
in alcohol and imbedded in celloidin. The sections obtained in this way 
are stained with the hsematoxylin solution (1 grm. lisematoxylin dissolved 
in a small quantity of C 2 H 6 0 and 100 grm. of 2 per cent, acetic acid). 
The staining is effected in from one to three hours. The sections are 
then placed in a mixture of 100 ccm. of saturated solution of lithium car- 
bonate, and 10 ccm. of 1 per cent, solution of red prussiate of potash. 
* Anat. Anzeig., v. (1890) pp. 524-6. f T. c., pp. 519-24. 
% See this Journal, 1890, p. 115. 
