426 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
appear of a blue-black colour, while the cells are yellow or yellowish 
brown. The specimens hardened in Muller’s fluid and afterwards in 
alcohol were imbedded in celloidin. The sections were then placed 
for twenty-four hours in a paraffin stove at a temperature of 45° in 
Kultschitzky’s haematoxylin solution (haematox. 2 g., alcohol abs. q. s. ad 
solut., acetic acid, 2 per cent., 100 ccm.). 
After this the sections were immersed in Muller’s fluid, and then 
treated with 1/4 per cent, permanganate of potash, after which they 
were decolorized in acidum oxalicum 1*0, kalium sulfurosum 1*0, 
aq. dest. 200 ’0. Then, having been washed in water, they were dehy- 
drated, cleared up, and mounted. 
The second method stained beautifully the' protoplasmic process of 
Purkinje’s corpuscles in the cerebellum. In this the procedure con- 
sisted in hardening and sectioning as before, and then using the following 
mordant: — Vanadium chloratum, 10 per cent., 2 parts; aluminium 
aceticum liquidum, 8 parts. Herein the sections remained for twenty- 
four hours, they were then washed for 5-10 minutes in water, and then 
having been stained with haematoxylin as in the first method, were 
decolorized with Weigert’s fluid. 
In the third method the pieces were hardened by Kultschitzky’s 
method,* and after-hardened in 96 per cent, spirit. The section mass 
was imbedded in either celloidin or paraffin. The sections were then 
immersed for twenty-four hours in the following mordant: — Vanadium 
chloratum, 10 per cent., 2 parts ; aluminum aceticum liq., 8 per cent., 
8 parts. After having been washed for ten minutes in water the sections 
were placed in the haematoxylin for twenty-four hours. The staining 
was then differentiated with 80 per cent, alcohol to every 200 parts of 
which 1 part HC1 was added. 
When they assumed a bluish-red hue the acid was removed in weak 
spirit, after which the sections were dehydrated in absolute alcohol, 
cleared up in origanum oil, and mounted in balsam. 
By this method the large cells of cerebrum and cerebellum, their 
protoplasmic processes, axis-cylinders, and the glia-cells were well 
stained. 
Staining Osseous Tissue by Golgi’s Method. - ]* — Sig. V. Tirelli found 
that Golgi’s method was suitable for studying osseous tissue, and very 
advantageous for flat bones ; for example, the skull bones of an almost 
mature rabbit embryo. Against a yellow background the bone-corpuscles 
stand out stained more or less dark-brown, the staining in the centre 
of the elements being less pronounced than at the periphery or in the 
processes. 
The reaction does not affect every individual element, but occurs 
usually in groups of five to thirty ; and this is an advantage rather than 
not, since it allows the recognition of delicate details of structure. 
Impregnating Brain of Amphibia by Golgi’s Method.f — Herr A. 
Oyarzun calls attention to the fact that in Ramon y Cajal’s modification 
* See this Journal, 1888, p. 510. 
t Atti R. Accad. Lincei Roma — Rendiconti, vi. (1890) pp. 24-G. 
X Arch. f. Mikr. Anat., xxxv. (1890) pp. 380-7 (2 pis.). See Zeitschr. f. Wiss 
Mikr., vii. (1891) p. 509. 
