ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
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parations. Fresh lymph preparations were examined in hanging drops. 
Dry preparations were made by fixing lymph films on cover-glasses with 
sublimate or sublimate and alcohol, and stained with anilin dyes (methy- 
len-blue, gentian- violet, fuchsin), haematoxylin, and picrocarmin. 
Infection Experiments with Sarcosporidia.* — Dr. Th. Kasparek 
obtained some fresh Miescher’s tubes from the oesophagus of recently 
killed sheep, and after having cleaned them with sublimate alcohol and 
ether, opened them with scissors. The contents thus obtained (about 
1/2 ccm.) were injected subcutaneously into the muscles of the back of 
guinea-pigs. In about 36 hours the first animal died. Blood prepara- 
tions were stained with methylen-blue, and a few spindle-shaped cells 
having some resemblance to sickle-shaped germs were observed. The 
blood of the second guinea-pig was examined four hours after the injec- 
tion. Some drops of blood obtained from the aural veins were stained 
with methylen-blue serum (a mixture of equal parts of Loefiler’s alco- 
holic methylen-blue solution, and of blood serum, a small quantity of 
thymol being added). These blood preparations showed some well 
stained Sporozoa, lying free between the blood-corpuscles. Twelve 
hours after, no sickle-germs were observable, though there were many 
cells having some resemblance thereto, but not clearly distinguishable 
from leucocytes. The animal died in 24 hours, but nothing was found. 
Further experiments gave the same results, and they are interesting as 
showing that Sporozoa, when injected subcutaneously, may reach the 
blood circulation, in which they very soon alter their shape. Whether 
this alteration of shape is a precursor of death, or a normal change pre- 
paratory to another stage, remains to be shown. 
Bovine Haematuria.j — Sig. F. Sanfelice and Sig. L. Loi had the 
opportunity of observing ten cases of haematuria in cattle in Sardinia, 
where this disease is prevalent. The same microbes were always found 
in the blood-corpuscles. They are round or pear-shaped. In some 
corpuscles only one organism is observed, in others two joined together 
like the figure 8. They stained well with anilin dyes, and some better 
along the margin. Subcutaneous inoculation of a cow reproduced the 
organism after 16 days. The authors think the organism is identical 
with that found by Babes in bovine haematuria, and by Smith and Kil- 
born in Texas fever. 
Lipoma Protozoon.J — Dr. Vedeler describes a Protozoon which in- 
habits tumours composed of adipose tissue. They are spheroidal forms, 
with doubly contoured investing membrane and opaque contents ; or the 
contents are finely granular, and then a nucleus and nucleolus are visible. 
In some examples the specimens appeared to show early spore-forma- 
tion. 
Mode of origin of the different varieties of the Malaria Para- 
sites of the Irregular or iEstivo-Autumnal Fever.§ — According to Herr 
N. Sacharoff, all the observed varieties of the parasites of the irregular 
malarial fever represent a single form of the malaria parasites — the para- 
* Centralbl. f. Bakteriol. u. Parasitenk., l ta Abt., xviii. (1896) pp. 327-30. 
f Moderno Zooiatro, 1895, p. 344 (1 pi.). See Centralbl. f. Bakteriol. u. Para- 
sitenk., l te Abt., xix. (1896) pp. 295-6. 
% Centralbl. f. Bakteriol. u. Parasitenk., l t0 Abt., xix. (1896) pp. 274-6 (2 figs.). 
§ Tom. cit., pp. 268-73. 
1896 P 
