356 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
Spirillum TJndula magus in a medium the basis of which is meat-agar, the 
adjuncts being pepton, ammonium sulphate, and potassium nitrate. 
The last three ingredients are added in the proportion of 0*1 per 
cent. 
Ftg. 70 . 
Cultivation of Amoebae on Solid Media.* — Prof. A. Celli has found 
that the most suitable medium for the cultivation of Amoebae is Fucus 
crispus, made like agar with 5 per cent, of water, with or without bouillon, 
and always strongly alkalinised (to 10 ccm. of the medium, 1 ccm. of 
a solution N/10 of caustic potash, or 4-5 ccm. of a saturated solution of 
sodium carbonate may be added). Hanging drops are better without the 
bouillon. It was found to be practically impossible to obtain Amoeba 
colonies free from bacteria, although it was quite easy to obtain pure 
Amoeba cultures, that is to say, isolation of the species and varieties. 
Isolation was effected by cultivating the Amoeba material in the Fucus 
medium in Petri’s capsules, and then waiting for cyst formation ; culti- 
vations were then made in hanging drops from the ripe cysts. From these 
it was easy to obtain a single species or variety. In cultures from intestinal 
contents Infusoria are often present, but are easily got rid of by 1 to 3 
transfers. The apparatus required for examining Amoeba is a hot stage, 
or, better still, a thermostat-Microscope. By the above method, which 
is much simpler than that of Beyerinck, pure cultivations of Amoeba 
guttula, oblong a, undulans, coli, spinosa, diapliana , vermicularis, and 
arbor escens were obtained, and observed throughout the course of their 
life-history. 
Cultivating Protozoa on Solid Media. f — Dr. F. Schardinger ob- 
tained pure and bacteria-free cultures of Protozoa by incubating samples 
of the material in hay-infusion at 37°. Next day there were numberless 
swarmers on the surface, and from this surface the condensation water 
of an oblique hay-agar tube was infected. By the third day of incuba- 
tion at 37°, bacteria, cocci, and much larger forms were observable. 
Fresh nutrient material was inoculated from a place where these larger 
* Centralbl. f. Bakteriol. u. Parasitenk., l te Abt., xix. (1896) pp. 536-8. 
t Tom. cit., pp. 538-45. 
