698 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
In tbo first and simple form shown in figs. Ill and 112 the cutting 
blade is provided with two holes A A, in which are screwed two double 
clamps serving to fasten the brass wire B. By bending the wire and 
altering its position in the clamping screws, the distance between wire 
and blade can be regulated. 
In the more complicated form shown in figs. 113 and 114 the rais- 
ing or lowering of the stretcher c is regulated by the screw b, while the 
small handle a allows the wire to be raised so as to remove the sections 
from the knife. 
(4) Staining and Injecting. 
Application of Aniiin Mixtures for the Tinctorial Isolation of 
Tissue-Elements.* — Dr. P. G. Unna recommends Weigert’s method for 
staining fibrin and bacteria as being specially suitable for dermatological 
specimens. While in this method the decolorising effect of the aniiin 
was modified by the presence of xylol, and thus certain tissue-elements 
were preserved from altogether losing their colour, the author has 
employed the decolorising effect in a new direction. The pigments 
used were gentian-violet, metliylen-blue, and polychrome methylen-blue ; 
and for decolorising, six groups of aniiin mixtures were employed : — 
(1) mixtures of aniiin with acids ; (2) with salts and double salts ; 
(3) with acid pigments ; (4) with acid pigments and acids ; ' (5) with 
iodine ; and (6) with carbolic acid. The effect of these mixtures as 
regards their staining or decolorising action on the various normal and 
pathological tissues, and also on agar and blood-serum, are fully dis- 
cussed. The more important formulae for staining Hyphomycetes and 
Schizomycetes, and sections of normal and pathological cutaneous tissues, 
are also given. 
Retina of Selachian s.f — Herr L. Neumayer has followed Ramon y 
Cajal’s example in trying methylen-blue coloration as well as chrom- 
osmic-silver impregnation in his study of the retina in Selachians. But 
after some entirely negative results with the former method, he adhered 
to metallic impregnation. 
Staining of Sexual Nuclei.:]: — Miss E. Sargant has adopted with 
success the following methods ( Lilium Martagon). For fixing : Flem- 
ming’s solution, viz. : — 3 ccm. of 10 per cent, chromic acid in water, 
8 ccm. of 1 per cent, osmic acid, 2 ccm. glacial acetic acid, 27 ccm. 
absolute alcohol. The ovaries were then placed in 0*5 percent, aqueous 
solution of chromic acid for 18-24 hours ; then washed and placed 
successively in 30 per cent., 50 per cent., and 70 per cent, alcohol, at 
intervals of 24 hours, and finally removed to methylated spirit. For 
imbedding and cutting, bergamot oil was used as a penetrating agent 
(Heidenhain’s process). For staining : — (1) Flemming’s orange method 
for material fixed in Flemming’s solution. The sections were left about 
30 hours in 1 per cent, solution of safranin in absolute alcohol diluted 
with its own bulk of water ; then washed out in 50 per cent, alcohol, 
* S.A. aus Monatshefte f. prakt. Dermatologie, xxi. (1895). See Centralbl. f. 
Bakteriol. u. Parasitenlc., xx. (1896) p. 406. 
t Arcli. f. Mikr. Anat., xlviii. (1896) pp. 83-111 (25 figs.). 
% Ann. Bot., x. (1896) pp. 473-5. 
