ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
125 
germs of these diseases grow quite characteristically. For tuberculosis 
the above-mentioned solution of salts containing 7 per cent, glycerin 
and 1 per cent, pepton, and for solid media this latter liquid without 
pepton plus 1 per cent, agar was used. The growth of the germ in 
both these media is rapid and characteristic. 
For the cultivation of glanders the mode of preparation was the same 
as for tuberculosis except that only 5 per cent, glycerin was used, the 
solution was allowed to remain slightly acid instead of being neutral- 
ized. and no pepton was added. 
The solution of salts used for these media when first prepared is 
alkaline, but by boiling it can be rendered neutral or acid as ammonia is 
given off. 
Inoculation Apparatus for Rats and Mice.* — Dr. K, Muller uses 
an apparatus for fastening down rats and mice when required for inocu- 
lation purposes. It merely consists of a board with a couple of spring 
clamps, one at either end ; one of these serves to fix the tail, and the 
other the forceps which hold the neck. To ensure perfect stillness, a 
second pair of forceps applied to the loose skin about the neck or lower 
jaw, and fetters for the feet are necessary. 
Hot-water Thermostat with Automatic Regulator.! — Dr. E. A. 
Schepilewsky has devised a thermostat which is heated by a current of 
hot water, and in which the desired temperature is maintained by means 
of an automatic regulator. In general plan the apparatus somewhat 
resembles that of Kurtschinski,| and the regulator is not unlike that of 
Heydenreich. 
The apparatus consists of three parts, the thermostat A, the boiler B, 
and the regulator C, O'. In the water-jacket of the thermostat is a worm 
(only partially represented in the illustration, fig. 14) made of thin 
eopper tubing with a diameter of 7-8 mm. The boiler is a copper vessel 
incased in asbestos. At its top are two apertures a and b ; the former, 
always open, is for pouring in water, the latter is closed by a cork 
through which passes a glass tube with an elbow. The lower end of 
the tube reaches belo w the water-level in the boiler, and its upper end is 
connected with a rubber tube d , through which the water passes to the 
thermostat. At the bottom of the boiler is the short tube c , connected 
with the rubber tube e e, by which the water is returned from the 
thermostat to the boiler. The source of heat is a petroleum lamp. The 
regulator consists of two parts, a glass reservoir C, and a set of tubes C' 
fixed to a piece of board. The Y-piece ///is intercalated in the 
course of the rubber tube d. It is a glass tube with a diameter of 
6-7 mm. the arms of which are set at an angle of about 80° while the leg i, 
8 cm. long and 3 mm. in diameter, is connected by means of a rubber 
tube o o with the glass cylinder K (8 mm. in diameter and 10-12 cm. 
long). The upper end of K is connected with the reservoir C, by a 
thick rubber tube l, having an internal section of only 1-2 mm. The 
reservoir C, made of thin glass, is 2 cm. in diameter, and 24 cm. long. 
Passing through its upper end and reaching nearly to the bottom is a 
stout glass tube, the external extremity of which is T-shaped, one arm 
* Centralbl. f. Bakteriol. u. Parasitenk., xiii. (1893) pp. 596-7 (1 fig.). 
f Op. cit., xiv. (1893) pp. 131-8 (1 fig.). X See this Journal, 1893, pp. 384-5. 
