ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
407 
water, 100 ccm. 85 per cent, spirit, and 50 grm. glycerin. This was 
modified by the author in the following way j instead of 85 per cent, 
spirit, 95 per cent, was used, and then with the original reducing fluid 
two different solutions made. One of these consisted of equal parts of 
Kolossow’s fluid and a mixture of 8*0 pyrogallic acid, 3*0 citric acid, 
17*0 natrum sulfurosum, and 150*0 distilled water. The other was 
composed of 10 ccm. of the Kolossow solution, 3 ccm. spirit, 2 ccm. 
20 per cent, tannin, and 1 ccm. glycerin. 
Microchemical Staining Reactions of Mucin.* — Besides the already 
known microchemical staining reactions for mucin, Sawtschenko pro- 
poses the following : — Hardening in sublimate ; staining with borax- 
carmine followed by Gram. The smallest particles of mucin, the mucous 
degeneration of cells of mucous glands, the contents of cancer cells 
simulating Coccidia and designated by some Sporozoa, assume when 
stained by this method a deep violet-blue hue, while the capsules of these 
bodies, as well as the whole of the protoplasm of the cells, are of a pink 
or red hue. 
Fig. 34. 
Weigert’s Fibrin Method.| — Dr. S. Ehrmann points out that his 
previous work on pigmentation has not been duly recognized by 
Kromayer in his recent application of Weigert’s fibrin-method. What 
is new in Kromayer’s communication is the 
conclusion that the pigment-lines in the epi- 
thelial cells represent a breaking-down of the 
protoplasmic fibres, and this, Ehrmann says, 
is not true. He believes that the blood-pig- 
ment is the material out of which the cell-pro- 
toplasm forms the pigment just as the plas- 
modium malarias forms melanin. Ehrmann 
discusses Weigert’s method in some detail and 
has further criticism of Kromayer’s work. 
( 5) Mounting-, including Slides, Preservative 
Fluids, &c- 
Slide-holder 4 — Dr. Eabre-Domergue has 
devised a contrivance for holding a number 
of slides, so that the preparations thereon 
may be treated by various fluids or reagents 
without disturbance, and with little trouble 
and loss of time. 
It is a disc of copper, 40 mm. in diameter, 
on the surface of which are soldered six metal 
clips. The clips are simply brass plates bent 
on themselves into a U-shape. In these clips 
the slides are slipped and fixed with Mayer’s 
albumen and heat. The slide-holder is then transferred successively to 
a series of bottles containing the various stains and re-agents. These 
bottles are 85 mm. high and 35 mm. broad, and are placed in a common 
wooden support. 
* Centralbl. f. Bakteriol. u. Parasitenk., xv. (1894) p. 485. 
t Arch. f. Mikr. Anat., xliii. (1894) pp. 79-95. 
X Annales de Micrographie, vi. (1894) pp. 84-6 (1 fig.). 
