610 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
Experimental Production of Bovine Peripneumonia.* * * § — M. S. Ar- 
loing has produced typical bovine pneumonia in cattle by injecting pure 
cultivations of Pneumobacillus liquefaciens bovis into the lungs. There 
were the usual areas of pneumonic consolidation in the lungs, the 
fibrinous pleurisy and swelling of the lymphatic glands. The dose 
injected was 5 or 10 ccm. of cultures from the fourth to tenth genera- 
tion, the injection being repeated for several successive days. 
The source of the cultivations was a subcutaneous swelling near the 
root of the tail. When transferred to peptonized bouillon, and after- 
wards to gelatin plates, two different bacilli were isolated. One of these 
liquefied, the other did not ; both were provided with numerous cilia, 
the non-liquefying was usually shorter than the other. Only the liquefy- 
ing form ( Pneumobacillus liquefaciens bovis) was used in the experiments. 
Secondary Foci in Experimental Diphtheria.^ — Drs. A. C. Abbott 
and A. A. Ghriskey describe very minute yellowish lens-shaped foci in 
the omentum of guinea pigs after subcutaneous injection of pure culti- 
vations of diphtheria. On microscopical examination these deposits 
were found to consist of polynuclear leucocytes, the majority of which 
contained diphtheria bacilli. In the course of experiments it was found 
that injection of bouillon cultures, or of suspensions of bacilli in physio- 
logical salt solution into the testicles, gave satisfactory results. The 
amount injected varied from 0 ■ 4 to 0'7 ccm. 
The method adopted for demonstrating the bacilli was to stain the 
tissues first with aqueous Bismarck brown solution, then wash in alcohol 
and stain with Gram’s method. The bacilli were also stained with 
Loffler’s alkaline methylen-blue solution. The authors suggest that the 
bacilli were carried through the lymphatic vessels by wandering cells. 
Bactericidal Power of Dog’s Blood, and its Richness in Leuco- 
cytes.J — Dr. J- Havet finds from experiments made on dogs that the 
white blood corpuscles almost or altogether disappear when microbic 
products are injected into the blood, and this disappearance is coincident 
with partial or total loss of bactericidal power. As the leucocytes re- 
appear the bactericidal power returns pari passu. When living cultures 
are injected into the tissues, the stage of hypoleucocytosis is accompanied 
by a diminution of the bactericidal power, and conversely hyperleuco- 
cytosis by increase. 
This increase is due to the presence of leucocytes in greater 
numbers, and is not the result of some newly acquired property of the 
serum. No fixed relationship between richness in leucocytes and bacte- 
ricidal power can be established, for the leucocytes may be weakened 
either by a previous digestion of microbes, or by the microbic poison. 
Leucocytes, in case of two kinds of organisms, may incorporate both or 
only one, according to the medium in which they are acting. 
Physiological Effect of the Soluble Products of Pyogenic Sta- 
phylococci^ — Sig. J. Salvioli, after finding that intrajugular injection of 
* Comptes Bendus, cxix. (1894) pp. 143-6. 
t Johns Hopkins Hospital Bulletin, iv. (1893) pp. 29-31 (6 figs.). 
t La Cellule, x. (1894) pp. 221-47. 
§ Berlin. Med. Wochenschr., 1893, No. 13. See Centralbl. f. Bakteriol. u. 
Parasitenk., xv. (1894) p. 1007. 
