SUMMARY OF CURRENT RESEARCHES. 639 
In the treatment of the brains and sections the author uses the follow- 
ing process : — After previous injection with Muller’s liquid, to which 
one-quarter of its volumo of a 5 per cent, solution of lysol has been 
added, the brain is hardened and kept in a dark cupboard at the ordinary 
temperature of the room. Hardening in the hatching oven is to bo 
avoided. 
The pieces are dried with Jblotting-paper, and without washing are, 
after a short stay in absolute alcohol, immersed in photoxylin. They 
are then glued to a metal plate which is attached to a smaller piece of 
wood fitting into the object-clamp. The use of a metal plate is indis- 
pensable, as wooden plates would warp in consequence of the prolonged 
immersion in the bath. 
In the sectioning, the sections sink in the water and are caught 
on a sheet of paper with the help of a fine brush. 
For staining the sections the author has resorted to the principle 
described by Oregia,* which consists in bringing the section on to a 
glass plate coated with a mixture of candied sugar and dextrin. The 
section adheres to the layer of sugar and the paper can be removed. 
The preparation is then dried with fine blotting-paper and afterwards 
covered with a thin layer of photoxylin, which on drying is pressed 
against the section by means of a roller. The plate is then placed in 
water, when the sugar dissolves, setting free the section with its adhering 
photoxylin backing; such sections can then be easily stained with 
hsematoxylin, since they can be passed through the various liquids 
without injury. 
Quick Double-staining Method for Examination of Blood and 
Tissues.f — Dr. Inghilleri’s method of double-staining depends on the 
mordant and fixative properties possessed by absolute alcohol, ether, and 
chloroform. The preparations are to remain for just 30 minutes in any 
of these fluids, but not longer, otherwise their sensitiveness to staining 
reagents is diminished. The procedure is as follows : — The cover-glass 
or section is first placed in chloroform for 30 minutes, and then in a 
mixture of 40 parts 1 per cent, eosin in 70° alcohol and 60 parts of a 
saturated aqueous solution of methylen-blue. The solution should be 
warmed for 2 or 3 minutes. 
The method is very suitable for the study of phagocytosis and of 
malaria parasites. 
Demonstrating Nucleated Red Corpuscles.!— M. Timofeyewski has 
found that by injecting a solution of sodium chloride into animals 
large numbers of nucleated red corpuscles appear in the blood soon after 
the injection. These nucleated globules are about the same size as the 
ordinary red corpuscle, but they contain a round, well-defined nucleus 
4-5 /x, in diameter, often placed excentrically. When stained by the 
Ehrlich mixture they are either black or assume a blue or greenish hue. 
The investing membrane of the nucleus is always quite distinct. Some- 
times the nuclei are freed and devoid of protoplasm. 
The solution injected consisted of sodium chloride exposed to the air 
* See Neurol. Centralbl., 1890. 
t Centralbl. f. Bakteriol. u. Parasitenk., xv. (1894) pp. 820-1. 
% Wratsch, 1894, No. 2. See Ann. de Micrographie, vi. (1894) pp84-6. 
2x2 
