698 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
darkly staining centre. It occasionally indents the nucleus. Multipli- 
cation appears to take place by simple division, and when examined 
fresh it shows amoeboid movements. In the early condition of vaccinia 
these bodies occur in the Malpighian layer, but after a few days are only 
seen at the edge of the pustule. The same organisms were found in 
sections of skin and mucosa of variolous patients. 
The tissue was fixed with saturated solution of sublimate, and after- 
wards hardened in alcohol. The sections were stained with hematoxylin, 
carmine, and lichtgriin, Ehrlich-Biondi, &c. The parasite was easily 
stained, but preferentially with protoplasmic dyes. 
To the foregoing Dr. S. M. Copeman * * * § replies that the bodies described 
cannot be considered organisms special to vaccinia, since they can be 
“ grown ” in the corneal epithelium of the rabbit. The author (and 
Dr. Klein as well) has also shown that small bacilli are always present 
in the early stages of the vaccine vesicle of man and of the calf. 
Parasites of Sarcoma.f— M. Gueynatz describes bodies which he 
has found in seven sarcomata. In position they are intranuclear, round 
or ovoid in shape, with a distinct outline but no definite membrane, and 
from 2-5 /x in diameter. These bodies have a decided affinity for red 
pigments, and contain slightly refracting granules varying in size from 
0*5-1 *5 jx. These bodies are most frequent at the growing edge of the 
tumour and are usually intranuclear, though occasionally they are seen 
in the cell-plasma or even free. As they persist during mitosis they are 
not nucleoli, nor do nucleoli contain bright refracting granules stainable 
with difficulty, nor are nucleoli found outside the nucleus. 
The tumours were placed directly after removal in Flemming’s fluid 
or pure spirit. The sections were stained with safranin and hasma- 
toxylin, or with some anilin dye and eosin. 
Prof. Petroff J has examined twenty-four sarcomata, and in all has 
found bodies very similar to those of carcinoma, although considerably 
smaller. They are usually located in the cell-plasma, and are rarely 
intranuclear or intercellular. In number, size, and arrangement they 
are extremely variable. Some are nucleated, in which case the nucleus 
stained green and the plasma yellow, while the nucleus of the tissue-cell 
stained blue. The cells containing these bodies are profoundly altered ; 
they are hypertrophied, the nucleus being pushed aside and its chromatin 
forming an irregular mass. The number of these bodies varied with 
the kind of sarcoma, i. e. they were sparse in the spindle or round-cell 
sorts, but in the myeloid were frequent. The pieces were hardened in 
Muller’s or Flemming’s fluid. The best stains were found to be car- 
bolized methylen-blue and anilin-yellow. 
Parasites of Cancer. — According to Dr. P. Foa§ the cancer-body 
consists of a nucleus surrounded by a thin layer of protoplasm and 
bounded by a doubly contoured capsule. This capsule is ofttimes finely 
and regularly striped, and the protoplasm so wrinkled that the whole 
body resembles a cockade or a rosette. These segments, however, cannot 
separate from another, and are not spores. The nucleus continues to 
* Op. cit., July 21, 1894, p. 157. 
f Wratsch, 1894, Nos. 8 and 9. See Ann. de Microgr., vi. (1894) pp. 289-98. 
t Gazette de Bptkine, Jan. 1894. See Ann. de Microgr., vi. (1894) pp. 287-8. 
§ Mitteil. XI. Internat. Med. Kongr. in Rom. See Centralbl. f. Bakteriol. u. 
Parasitenk., xv. (1894) pp. 81G-7. 
