ZOOLOGY AND BOTANY, MICROSCOPY, ETC. 
99 
Pure Cultures of Amoebae.* — M. J. Tsujitani lias been able to obtain 
pure cultures of amoebae on substrata containing bacterial products 
and dead bacteria. Three varieties of Amoeba lobosa were used, and 
were cultivated in company with bacteria in the usual manner. The 
most convenient bacterium was the cholera bacillus. From these mixed 
cultures amoeba cysts were obtained by dipping silk threads therein and 
* Centralbl. Bakt. u. Par., l ta Abt., xxiv. (1898) pp. GG6-70. 
II 2 
culture escape unhindered, but if necessary may be collected by 
inverting over the valve a eudiometer F filled with the boric acid 
solution. 
Fig. 18. 
Fig. 19. 
