348 
SUMMARY OF CURRENT RESEARCHES RELATING TO 
per cent, solution of potassium bichromate until they assume a violet 
colour. They are then washed in water, dehydrated in absolute alcohol, 
cleared in xylol, and mounted in balsam. By this method the mucigen 
granules are stained violet, and the rest of the cell blue. 
The mucous cells of salivary glands are not stained by this method. 
The preparations fade very quickly, but the method is valuable for the 
study of mucigen granules. 
Apparatus for Heating Staining Solutions.* — Dr. 0. Korn de- 
scribes with considerable amplitude an apparatus for holding a watch - 
Fig. 9.3. 
glass filled with staining solution over the flame. As the illustration 
shows (fig. 93), it much resembles a spoon, with a shallow wire-sieve 
bowl. 
(6) Miscellaneous. 
Simple Method for Detecting the Typhoid Bacillus.f — Herr Pior- 
kowski states that by means of the following procedure a certain 
diagnosis of enteric fever can be made from the stools within 20 hours. 
Healthy urine, of sp. gr. 1020, is collected for 2-3 days, by which time 
it has acquired an alkaline reaction. The urine, mixed with 0*5 per 
cent, pepton and 3 • 3 per cent, gelatin, is boiled for #n hour in a water- 
bath and then at once filtered, after which it is distributed into tubes. 
The tubes are plugged with cotton-wool, and steam sterilised at 100° for 
15 minutes. The sterilisation is repeated next for 10 minutes. Three 
tubes are inoculated with typhoid faeces ; the first with 2 loopfuls, the 
second with 4, and the third with 6-8. The plates are now laid, and 
when set must be kept at a temperature of 21°-2i°. 
By this procedure the yellowish-brown round colonies of B. coli and 
* Centralbl. Bakt. u. Par.. l te Abt., xxv. (1899) pp. 422-3 (2 figs.), 
t Tom. cit., p. 319. 
