16 
water must be kept free from fragments of the various organs 
of the adult, as these would soon decay and destroy the em- 
bryos, and it is therefore important to remove them as com- 
pletely as possible. After the mass has been chopped up as 
fine as possible, fill up the watch crystal with fresh sea water, 
stir it up, and then allow it to run into one of the smallest 
beakers, which has been nearly filled with sea water. As the 
water runs out of the watch crystal, be careful to allow as. 
few of the fragments as possible to run with it. 
ISTow fill up the watch crystal with water again, and stir 
and pour off as before, and repeat the process until nearly all 
of the male fluid has been washed out of the fragments and 
poured into the beaker. Stir the contents of the beaker for a 
short time, and then allow it to stand about five minutes, to 
allow any fragments to settle to the bottom, then pour the 
fluid, which should be quite milky, into another small beaker, 
leaving behind, to be thrown away, any particles which may 
have settled to the bottom. The male cells retain their full 
vitality for several hours after they have been mixed with sea 
water, so the beaker may be set aside to wait until the eggs 
are ready. The eggs swell up and break to pieces within a 
very few minutes after they are mixed with water, unless they 
are fertilized at once, so it is much better to add the eggs to 
a previously prepared mixture of male cells and water than it. 
is to put the eggs into the water to wait until the male fluid 
is got ready. 
Taking now one of the females, remove and chop up the 
ovary in the same way in another watch crystal, observing 
the same precautions in removing all portions of the body. 
Fill the w^atch glass with water, and stir and pour off into the 
beaker as before, giving the contents of the beaker a good, 
stirring after each lot of eggs is added, in order to diffuse 
them through the water at once, and thus insure the speedy 
contact of each of them with some of the male cells. 
Fill the crystal with water again, and stir and pour off,, 
and repeat until all the eggs have been washed out of the: 
fragments of the ovary. 
